Protein Info for Psyr_0086 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 639 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 46 to 62 (17 residues), see Phobius details PF00989: PAS" amino acids 92 to 198 (107 residues), 38.7 bits, see alignment E=3.2e-13 TIGR00229: PAS domain S-box protein" amino acids 92 to 208 (117 residues), 65.9 bits, see alignment E=3.8e-22 PF13188: PAS_8" amino acids 92 to 124 (33 residues), 28 bits, see alignment (E = 5.6e-10) PF08448: PAS_4" amino acids 93 to 200 (108 residues), 36.7 bits, see alignment E=1.5e-12 PF13426: PAS_9" amino acids 98 to 200 (103 residues), 60 bits, see alignment E=8.5e-20 PF08447: PAS_3" amino acids 109 to 194 (86 residues), 35.4 bits, see alignment E=3.8e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 209 to 370 (162 residues), 130.9 bits, see alignment E=3.7e-42 PF00990: GGDEF" amino acids 212 to 368 (157 residues), 150.7 bits, see alignment E=1.1e-47 PF00563: EAL" amino acids 389 to 624 (236 residues), 251.2 bits, see alignment E=3.3e-78

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_0086)

Predicted SEED Role

"GGDEF domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q500L2 at UniProt or InterPro

Protein Sequence (639 amino acids)

>Psyr_0086 diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) (Pseudomonas syringae pv. syringae B728a ΔmexB)
MSDYSRNALRTAVLYGLLSILWFATGSALLSFFVKDPARLAVGQQMIGYGWALLSTVMIF
IARDRLKAIVGGEALFERQQQDYDRLLLAAVVFDSTREGVLVSDAEGRIVHVNRAFVDIT
GYQADEVLGFNPSKFKSGRHGPEFYRQMYHSLQSAGQWSGEIWNRRKSGEIYPQWQTIRS
IKGEDGQPRHFVAVFSDITAIKRSEQELAQLAHYDALTELPNRLLLTDRISQALTSARSS
RRGCALLVVDLDNFRNINDSLGHNIGDEVLKAAAERLNALADSGMTVSRLGGDEFALLVK
NCTHMVHAAALAQQVINAFKAPYPIAGHQLFVTASIGISLFPGDALTAEQLLRNADSALF
KAKTDGREGFALYTEELTAQAQQRVQLASELRQAIEQDELRVFYQPVYDLVTGRICAVEA
LVRWQHPTRGMVSPGDFIPIAEQNGLIADIDAWVLNTACRQMQAWLAEGVSIASVAVNVS
SRLFSRGDVDRQVAEVLNETGLDPAFLELEVTESAVMEDPEQAIEQLHRLRELGLSLAID
DFGTGYSSLLRLKSMPVQKLKIDQGFVAGLPGDVDDIAIVRAVIALASSLGMRVLAEGIE
HANQASFLLSNGCELGQGYWFARPMAAQSIDWTHAPAFN