Protein Info for Psyr_0083 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Binding-protein-dependent transport systems inner membrane component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 273 transmembrane" amino acids 12 to 37 (26 residues), see Phobius details amino acids 66 to 85 (20 residues), see Phobius details amino acids 97 to 119 (23 residues), see Phobius details amino acids 135 to 155 (21 residues), see Phobius details amino acids 187 to 205 (19 residues), see Phobius details amino acids 241 to 265 (25 residues), see Phobius details TIGR02139: sulfate ABC transporter, permease protein CysT" amino acids 8 to 270 (263 residues), 434.4 bits, see alignment E=1.7e-134 TIGR00969: sulfate ABC transporter, permease protein" amino acids 10 to 268 (259 residues), 318.7 bits, see alignment E=3.2e-99 PF00528: BPD_transp_1" amino acids 75 to 270 (196 residues), 76.4 bits, see alignment E=1.2e-25

Best Hits

Swiss-Prot: 51% identical to CYST_SALTY: Sulfate transport system permease protein CysT (cysU) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02046, sulfate transport system permease protein (inferred from 99% identity to psp:PSPPH_0088)

MetaCyc: 51% identical to sulfate/thiosulfate ABC transporter inner membrane subunit CysU (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate transport system permease protein CysT" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q500L5 at UniProt or InterPro

Protein Sequence (273 amino acids)

>Psyr_0083 Binding-protein-dependent transport systems inner membrane component (Pseudomonas syringae pv. syringae B728a ΔmexB)
MSRRISPVIPGFGLTLGYTLVYLSLIVLIPLAAMFVHAAQLTWDQFWTIITAPRVIAALK
LSFGTAFYAALINGVIGTLLAWVLVRYTFPGRKVIDAMIDLPFALPTAVAGIALTALYAP
NGWVGQFAADLGFKIAYTPLGITLALTFVTLPFVVRTVQPVLADIPREVEEAAACLGARP
LQVFRHILVPALLPAWLTGFALAFARGVGEYGSVIFIAGNMPMKTEILPLLIMVKLDQYD
YTGATAIGVMMLVVSFILLLLINLLQRRIETPS