Protein Info for Psyr_0015 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Oxidoreductase alpha (molybdopterin) subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 787 TIGR01701: oxidoreductase alpha (molybdopterin) subunit" amino acids 21 to 766 (746 residues), 950 bits, see alignment E=4.4e-290 PF00384: Molybdopterin" amino acids 117 to 494 (378 residues), 71.9 bits, see alignment E=5.2e-24 PF01568: Molydop_binding" amino acids 654 to 761 (108 residues), 37.8 bits, see alignment E=1.7e-13

Best Hits

Swiss-Prot: 51% identical to YDEP_ECO57: Protein YdeP (ydeP) from Escherichia coli O157:H7

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 100% identity to psb:Psyr_0015)

Predicted SEED Role

"Anaerobic dehydrogenases, typically selenocysteine-containing" in subsystem Anaerobic respiratory reductases

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q500T3 at UniProt or InterPro

Protein Sequence (787 amino acids)

>Psyr_0015 Oxidoreductase alpha (molybdopterin) subunit (Pseudomonas syringae pv. syringae B728a ΔmexB)
MSESENSQAPLQGSQTDGTPAGGWAALSAVNKHLLHQHVLIKGNRTLMSMNKPGGFDCPS
CSWPDPKKPHTFEYCENGAKALAWESTKKRVTPEFFARHTVSELATWSDHELEAEGRITE
PMRYDAATDKYLPVTWDEAFAEIGEELRNLSDPWKLELYTSGRTSNEAAFLYQLFGRMYG
MANFPDCSNMCHETTTAALPESIGVGKGTTTLEDFESADAIFIIGQNPGTNSPRMMSELH
AAARRGASIISFNPLRERALVRFAAPQDPRDMLSLHGVEISSQYHQVRIGGDMIALQGVC
KAVIEADDVAQREHLPRVLDVAFIEEHTHGFEQYADYCRQLPWDIIERHSGLTRQAIEEV
AAVYKRSKGVICCWGMGITQHKRGGDAMQQIINLLLLRGNIGKLGAGACPVRGHSNVQGD
RTMGIYEKAGEPFLASMSKVFGFEASRKKGHAVAETCEALLRGEVEALISMGGNFFRAIP
DTDVICPTVSRLKMTVLVNTKLNRSATVHGQKAFLLPCVARSERDLQNGVAQTITVEDSM
SMVHGSTGMLEPASKHLISEVAIIAGIAKATLAPNPKVDWDSYVGDYSRIRDKIEEVLPE
QFYDFNQRILEPGGFRLPNAASERKWDTESGKANFLFPEGIYDEDDTPPGAEHLQLMTIR
SHDQFNTTVYSNDDRYRDIFGDRMVVMLNPQDIERLGLKAGDYIEFQTALDPTTARRAAG
FKVIPYDVPQGCCAAYYPETNGLLPLANRDKHGNTPAAKSIPVNLVRMSSEGTQVALERR
GLIAAAS