Protein Info for PfGW456L13_979 in Pseudomonas fluorescens GW456-L13

Annotation: Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 270 TIGR00577: DNA-formamidopyrimidine glycosylase" amino acids 1 to 269 (269 residues), 294.8 bits, see alignment E=3e-92 PF01149: Fapy_DNA_glyco" amino acids 1 to 115 (115 residues), 128 bits, see alignment E=4.2e-41 PF06831: H2TH" amino acids 130 to 220 (91 residues), 84.3 bits, see alignment E=7.2e-28 PF06827: zf-FPG_IleRS" amino acids 242 to 270 (29 residues), 31.8 bits, see alignment (E = 1.5e-11)

Best Hits

Swiss-Prot: 97% identical to FPG_PSEPF: Formamidopyrimidine-DNA glycosylase (mutM) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K10563, formamidopyrimidine-DNA glycosylase [EC: 3.2.2.23 4.2.99.18] (inferred from 96% identity to pfl:PFL_5870)

MetaCyc: 56% identical to DNA-formamidopyrimidine glycosylase (Escherichia coli K-12 substr. MG1655)
DNA-formamidopyrimidine glycosylase. [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; RXN-21279 [EC: 3.2.2.23]

Predicted SEED Role

"Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23)" in subsystem DNA Repair Base Excision (EC 3.2.2.23)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 3.2.2.23 or 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QG07 at UniProt or InterPro

Protein Sequence (270 amino acids)

>PfGW456L13_979 Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23) (Pseudomonas fluorescens GW456-L13)
MPELPEVETTRRGIAPFLEGQRVSRVIVRERRLRWPIPEDLDVRLSGQRIVLVERRAKYL
LINAEVGTLISHLGMSGNLRLVEAGLPAAKHEHVDIELESGLALRYTDPRRFGAMLWSLD
PLNHELLIRLGPEPLTDLFDGERLFQLSRGRSMAVKPFIMDNAVVVGVGNIYATEALFAA
GIDPRREAKSISRARYLKLAIEIKRILAAAIERGGTTLRDFIGGDGQPGYFQQELFVYGR
GGEHCKVCGTGLREVKLGQRASVFCPRCQS