Protein Info for PfGW456L13_908 in Pseudomonas fluorescens GW456-L13

Annotation: Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 PF05157: MshEN" amino acids 70 to 168 (99 residues), 49.2 bits, see alignment E=5.3e-17 PF00437: T2SSE" amino acids 216 to 480 (265 residues), 309 bits, see alignment E=2.4e-96

Best Hits

KEGG orthology group: K02454, general secretion pathway protein E (inferred from 93% identity to pfo:Pfl01_5424)

Predicted SEED Role

"Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QKA6 at UniProt or InterPro

Protein Sequence (594 amino acids)

>PfGW456L13_908 Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB (Pseudomonas fluorescens GW456-L13)
MSVQIALQDRWLDLNDLLRELVAQGFISQDSAEHALNARRRNASLGQMHPLEFIASQQLD
DLSRPGKPLDLESLTLWLSRQAGQPYLRIDPLKINVAAVTPLMSYAFAQRHKILAVAIDR
EAVTVASAQPYVSGWEADLTHVLKLPIKRVVANPVDIQRLSVEFFRLAKSVTGANSADPH
VSTLGNFEQLLNLGASDQEPDANDAHIVNIVDWLFQYAFQQRASDIHIEPRREQGTVRFR
IDGVLHNVYQFPPQVTMAIVSRLKTLGRMNVAEKRKPQDGRVKTKTPDGGEVELRLSTLP
TAFGEKMVMRIFDPEVLLKDFDQLGFSADDLRRWQDMTRQPHGIILVTGPTGSGKTTTLY
TTLKKLATPEVNLCTIEDPIEMVEPAFNQMQVQHNIDLTFAAGVRALMRQDPDIIMIGEI
RDLETAEMAIQAALTGHLVLSTLHTNDAPSAISRLLELGVPHYLIKATVLGVMAQRLVRT
LCPHCKAPLTLGEDDWQTLTRPWQAPLPANAQRAIGCVECRDTGYRGRAGVYEIMQLSDG
IKALISPDTDLLAVRRQAFKEGMRSLRLSGAQKVAAGLTTIEEILRVTPQSEQN