Protein Info for PfGW456L13_904 in Pseudomonas fluorescens GW456-L13
Annotation: Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 94% identical to GCSP2_PSEPF: Glycine dehydrogenase (decarboxylating) 2 (gcvP2) from Pseudomonas fluorescens (strain Pf0-1)
KEGG orthology group: K00281, glycine dehydrogenase [EC: 1.4.4.2] (inferred from 82% identity to avn:Avin_47260)MetaCyc: 66% identical to glycine decarboxylase (Escherichia coli K-12 substr. MG1655)
Glycine dehydrogenase (decarboxylating). [EC: 1.4.1.27, 1.4.4.2]
Predicted SEED Role
"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)
MetaCyc Pathways
- folate transformations III (E. coli) (9/9 steps found)
- folate transformations II (plants) (10/11 steps found)
- glycine biosynthesis II (3/3 steps found)
- glycine cleavage (3/3 steps found)
- glycine degradation (3/3 steps found)
- folate transformations I (9/13 steps found)
- photorespiration I (6/9 steps found)
- photorespiration II (6/10 steps found)
- photorespiration III (5/9 steps found)
- reductive glycine pathway of autotrophic CO2 fixation (5/9 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.27, 1.4.4.2
Use Curated BLAST to search for 1.4.1.27 or 1.4.4.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A293PXA3 at UniProt or InterPro
Protein Sequence (957 amino acids)
>PfGW456L13_904 Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2) (Pseudomonas fluorescens GW456-L13) MSQLPSLSQLRDPNAFLRRHLGPDAAEQQAMLDSLGLGSRVDLIEQTVPPGIRLNRPLDL PPALDEEAALAKLRGYAEQNQVWTSLIGMGYHGTLTPAVILRNVLENPGWYTAYTPYQPE IAQGRLEALLNFQQLTIDLTGLELANASLLDEATAAAEAMALAKRVAKSKSNLFFVDENC HPQTISVVQTRAEGFGFDLIVDTVDNLKRHQVFGALLQYPDTHGEIHDLRPLIDHLHAQQ ALACVATDLLSLLLLTPPGELGADVVFGSSQRFGVPMGYGGPHAAFFASRDEYKRAIPGR IIGVSKDARGNTALRMALQTREQHIRREKANSNICTAQVLLANIASFYAVYHGPEGLKRI AQRVHRLTCILATGLERNGIVRVNRHFFDTLTLEVGGTQTAIIESARAAQINLRILGRGQ LGLSLDETCDEGTVAKLFDVFLGADHGLSIDDLDAEVLPGGIPQGLARTSPYLRHPVFSA HHSETEMLRYLKQLENKDLALNQSMIPLGSCTMKLNATSEMIPITWPQFANLHPFVPKEQ AAGYGLMIEELERWLCAITGFDAICMQPNSGAQGEYAGLLAIRKYHESRQQGGRDICLIP SSAHGTNPASAQMAGMRVVIVECDEAGNVDLDDLKEKAAQAAEKLACLMATYPSTHGVYE EGISEICEVIHQHGGQVYMDGANLNAQVGLARPADIGADVSHMNLHKTFCIPHGGGGPGM GPIGVRAHLAPFVANHPVVPIDGPHPQNGAVSAAPWGSASILPISWMYIAMMGPQLADAS EVAILAANYLARHLSGAFPVLYTGRNERVAHECILDLRPLKALTGITEEDVAKRLMDYGF HAPTMSFPVPGTLMVEPTESESKAELDRFIGAMLSIRAEINEVQNGNWPAEENPLKGAPH TLADITGVWERPYSIVQAVTPDAHTKAHKYWPVVNRVDNVYGDRNLFCACVPVDDYR