Protein Info for PfGW456L13_5128 in Pseudomonas fluorescens GW456-L13

Annotation: Glycerol kinase (EC 2.7.1.30)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 522 PF00370: FGGY_N" amino acids 9 to 257 (249 residues), 148.5 bits, see alignment E=2.7e-47 PF02782: FGGY_C" amino acids 267 to 461 (195 residues), 103.7 bits, see alignment E=1.1e-33

Best Hits

KEGG orthology group: None (inferred from 86% identity to pfl:PFL_2769)

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.30

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QXB8 at UniProt or InterPro

Protein Sequence (522 amino acids)

>PfGW456L13_5128 Glycerol kinase (EC 2.7.1.30) (Pseudomonas fluorescens GW456-L13)
MDNHPNKRYLLAIDNGTQSVRALLFDLQGNLLGKGKVELQAYYSTQPGWAEQDPEYYWAK
LGEACQQLWEQTGIDRSQIAGVSLTTQRGTVINVDAQGKALRPAILWLDQRQAEVKGGIK
GPWGWLFKLVGAQATVDYFRAQAEANWIARHQPEVWAATDKFLLLSGFLTHRLCGRFVDS
VGCCVGYLPFDFKRLRWAAPSDWKWQALAVRPEQLPSLHKPGETLGYISAEASRHTGIPE
GLPLIAAGADKACEVVGSGVVDPGTVCLSYGTTATITSTRSRYLEIVPLIPPYPSAVPDQ
YNCEVMIYRGYWMVSWFKNEFGLREMQQAKAQGVEPEQLFDALVDAVPPGSMGLMLQPYW
SPGIREPGVEAKGAMIGFGDVHTRAHIYRAILEGLAYALRQGMEKIEKRSRVSITRLRVA
GGGSQSDAAMQLTANIFGLPAERPHVYEASGLGAAICCAVGLGLHADFPTAMAAMTRVGA
VFMPQPEAQQIYERLYKEVYLRMYRQLKPLYQSIRKITGYPA