Protein Info for PfGW456L13_505 in Pseudomonas fluorescens GW456-L13

Annotation: HlyD family secretion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details PF00529: CusB_dom_1" amino acids 21 to 268 (248 residues), 27.2 bits, see alignment E=6.2e-10 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 46 to 233 (188 residues), 117.8 bits, see alignment E=2.5e-38 PF16576: HlyD_D23" amino acids 46 to 238 (193 residues), 47.3 bits, see alignment E=3.2e-16 PF13533: Biotin_lipoyl_2" amino acids 48 to 94 (47 residues), 59.4 bits, see alignment 4.5e-20 PF13437: HlyD_3" amino acids 155 to 238 (84 residues), 52.5 bits, see alignment E=1.6e-17

Best Hits

Swiss-Prot: 41% identical to YDHJ_ECOLI: Uncharacterized protein YdhJ (ydhJ) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 93% identity to pfo:Pfl01_0173)

MetaCyc: 38% identical to aromatic carboxylic acid efflux pump membrane fusion protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-233

Predicted SEED Role

"HlyD family secretion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QIH1 at UniProt or InterPro

Protein Sequence (295 amino acids)

>PfGW456L13_505 HlyD family secretion protein (Pseudomonas fluorescens GW456-L13)
MKKPFLTLGRVVLTLLIVSFAVVVVWRMVMYYMFAPWTRDGHIRADIVQIAPDVSGLIQQ
VEVRDNQLVKRGQVLFSIDPDRFKLALRQAKAAVADREENLAQAQREAKRNKGLGNLVPG
EQLEESQSKVARAQVALMEAQVAVDSAQLNLDRSVIRSPVDGYVNDRAPRPQEFVSAGRP
VLSVVDSNSFHIDGYFEETKLNGIHIGQSVDIRVIGDNARLRGHVESIVAGIEDRDRTSG
NNLLPNVNPAFSWVRLAQRIPVRIAFDDVPDDFRMIAGRTATVSIIDDQQLEPAQ