Protein Info for PfGW456L13_4962 in Pseudomonas fluorescens GW456-L13

Updated annotation (from data): Homogentisate 1,2-dioxygenase (EC 1.13.11.5)
Rationale: Specifically important for utilizing L-Phenylalanine. Automated validation from mutant phenotype: the predicted function (1.13.11.5) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: Homogentisate 1,2-dioxygenase (EC 1.13.11.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 TIGR01015: homogentisate 1,2-dioxygenase" amino acids 10 to 428 (419 residues), 637 bits, see alignment E=6.8e-196 PF20510: HgmA_N" amino acids 11 to 276 (266 residues), 396.2 bits, see alignment E=7.3e-123 PF04209: HgmA_C" amino acids 277 to 428 (152 residues), 264.4 bits, see alignment E=2.8e-83

Best Hits

Swiss-Prot: 94% identical to HGD_PSEPF: Homogentisate 1,2-dioxygenase (hmgA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00451, homogentisate 1,2-dioxygenase [EC: 1.13.11.5] (inferred from 94% identity to pfo:Pfl01_0910)

Predicted SEED Role

"Homogentisate 1,2-dioxygenase (EC 1.13.11.5)" in subsystem Homogentisate pathway of aromatic compound degradation (EC 1.13.11.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293Q6T7 at UniProt or InterPro

Protein Sequence (434 amino acids)

>PfGW456L13_4962 Homogentisate 1,2-dioxygenase (EC 1.13.11.5) (Pseudomonas fluorescens GW456-L13)
MNPDSTTPALAYQSGFGNEFSSEALPGALPVGQNSPQKAPYGLYTELFSGTAFTMARSEA
RRTWMYRIQPSANHPAFFKLDRQLTGGPLGEVTPNRLRWNPLDIPAEPTDFIDGLVSMAA
NSGAEKPAGISIYNYRANRSMERAFFNADGEMLLVPELGRLRIATELGVLELEPLEIAVL
PRGLKFRIELLDPQARGYVAENHGAPLRLPDLGPIGSNGLANPRDFLTPVAAYENLQQPT
TLVQKFLGQLWACELNHSPLNVVAWHGNNVPYKYDLRRFNTIGTVSFDHPDPSIFTVLTS
PTSVHGLANLDFVIFPPRWMVAEKTFRPPWFHRNLMNEFMGLIQGEYDAKAEGFVPGGAS
LHSCMSAHGPDGETCTKAINAELKPAKIDNTMAFMFETSQVLRPSRFALDCPQLQNTYDA
CWATLPATFDPTRR