Protein Info for PfGW456L13_4840 in Pseudomonas fluorescens GW456-L13

Annotation: Ribonuclease III (EC 3.1.26.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 208 TIGR02191: ribonuclease III" amino acids 3 to 199 (197 residues), 214.2 bits, see alignment E=8.5e-68 PF14622: Ribonucleas_3_3" amino acids 3 to 118 (116 residues), 126.3 bits, see alignment E=1.3e-40 PF00636: Ribonuclease_3" amino acids 16 to 106 (91 residues), 90.5 bits, see alignment E=1.6e-29 PF00035: dsrm" amino acids 134 to 199 (66 residues), 41.7 bits, see alignment E=2.2e-14

Best Hits

Swiss-Prot: 98% identical to RNC_PSEPF: Ribonuclease 3 (rnc) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03685, ribonuclease III [EC: 3.1.26.3] (inferred from 98% identity to pfo:Pfl01_0994)

MetaCyc: 55% identical to RNase III (Escherichia coli K-12 substr. MG1655)
Ribonuclease III. [EC: 3.1.26.3]

Predicted SEED Role

"Ribonuclease III (EC 3.1.26.3)" in subsystem RNA processing and degradation, bacterial or Two cell division clusters relating to chromosome partitioning (EC 3.1.26.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.26.3

Use Curated BLAST to search for 3.1.26.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0F4TMN7 at UniProt or InterPro

Protein Sequence (208 amino acids)

>PfGW456L13_4840 Ribonuclease III (EC 3.1.26.3) (Pseudomonas fluorescens GW456-L13)
MVLALTHRSFAGRNNERLEFLGDAILNFVAGEALFERFPLAREGQLSRLRARLVKGETLA
VLARGFDLGEYLRLGSGELKSGGFRRESILADALEALIGAIYLDAGMEMARERVLAWLAG
EFEGLTLVDTNKDPKTRLQEFLQSRGCELPRYEVVDIQGEPHCRTFFVECEIILLNEKSR
GQGVSRRIAEQVAAAAALIALGVENGND