Protein Info for PfGW456L13_4835 in Pseudomonas fluorescens GW456-L13
Annotation: Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 66% identical to PUR4_IDILO: Phosphoribosylformylglycinamidine synthase (purL) from Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)
MetaCyc: 65% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]
Predicted SEED Role
"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
- superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
- 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
- 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.5.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A293QWG9 at UniProt or InterPro
Protein Sequence (1298 amino acids)
>PfGW456L13_4835 Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3) (Pseudomonas fluorescens GW456-L13) MLILRGAPALSAFRHSKLLEQLSQKVPAVSGLYAEFAHFAEVTGVLTGDEQQVLARLLKY GPSVPVQEPTGRLFLVLPRFGTISPWSSKASDIARNCGLAKIQRLERGIAFYVAGQFSDA EAQLIADGLHDRMTQIVLGNLEQAAGLFSHAEPKPLTAIDVLGGGRAALEKANTELGLAL AEDEIDYLVNAFVGLKRNPHDIELMMFAQANSEHCRHKIFNASWDIDGESQEKSLFGMIK NTYVMHSEGVLSAYKDNASVIVGSVAGRFFPDPETRQYGAVQEPVHILMKVETHNHPTAI APFPGASTGSGGEIRDEGATGRGAKPKAGLTGFTVSNLQIPGFEQPWEVPYGKPERIVTA LDIMIEGPLGGAAFNNEFGRPALTGYFRTFEQSITTPHGDEVRGYHKPIMLAGGMGNIRE EHVKKGEIVVGSKLIVLGGPAMLIGLGGGAASSMATGTSSADLDFASVQRENPEMERRCQ EVIDRCWQLGDKNPISFIHDVGAGGLSNAFPELVNDGDRGGRFELRNIPNDEPGMAPHEI WSNESQERYVLAVGPADFERFKAICERERCPFAVVGEATAEPQLTVTDSHFGNSPVDMPL EVLLGKAPRMHRSVVRENELGDDFDPSTLSIADCVERVLHHPAVASKSFLITIGDRTITG LVARDQMVGPWQVPVADVAVTATSFDVYTGEAMAMGERTPLALLDAPASGRMAIGETLTN IAASRIGKISDIKLSANWMSAAGHPGEDARLYDTVKAVGMELCPELGITIPVGKDSMSMA TRWNDEGVDKSVTSPMSLIVTGFAPVTDIRQTLTPQLRMDKGTTDLILIDLGRGQNRMGA SILAQVHGKLGKQAPDVDDAEDLKAFFAVIQGLNADGHLLAYHDRSDGGLLTSVVEMAFA GHCGLSLNLDGLAETSADIAAILFNEELGAVIQVRQDATPDILAQFSAAGLGDCVSVIGQ PINNGEIGITFNGDTVFEGQRRLLQRQWAETSYQIQRLRDNADCAEQEFDVLLEEENPGL SVKLSYDINQDIAAPYIKKGIRPQVAVLREQGVNGQVEMAAAFDRAGFNAIDVHMSDILA GRVDLNEFKGMVACGGFSYGDVLGAGEGWAKSALFNSRARDAFQGFFERNDSFTLGVCNG CQMMSNLHELIPGSEFWPHFVRNRSEQFEARVAMVQIQESNSIFLQGMAGSRMPIAIAHG EGHAEFASEEALLEADLSGCVAMRFVDNHGKVTESYPANPNGSPRGITGLTSRDGRVTIM MPHPERVFRAVQNSWRSDDWNEDAPLMRMFRNARVWVN