Protein Info for PfGW456L13_4831 in Pseudomonas fluorescens GW456-L13

Updated annotation (from data): Glucosamine-6-phosphate deaminase [isomerizing], alternative (EC 3.5.99.6)
Rationale: Specifically important for utilizing N-Acetyl-D-Glucosamine. Automated validation from mutant phenotype: the predicted function (GLUCOSAMINE-6-P-DEAMIN-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: Glucosamine-6-phosphate deaminase [isomerizing], alternative (EC 3.5.99.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 transmembrane" amino acids 56 to 77 (22 residues), see Phobius details amino acids 306 to 323 (18 residues), see Phobius details PF01380: SIS" amino acids 44 to 158 (115 residues), 58.9 bits, see alignment E=2.3e-20 amino acids 198 to 281 (84 residues), 48.8 bits, see alignment E=3.1e-17

Best Hits

KEGG orthology group: K01504, glucosamine-6-phosphate isomerase [EC: 3.5.99.6] (inferred from 94% identity to pfo:Pfl01_1005)

Predicted SEED Role

"Glucosamine-6-phosphate deaminase [isomerizing], alternative (EC 3.5.99.6)" in subsystem Chitin and N-acetylglucosamine utilization or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 3.5.99.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QW53 at UniProt or InterPro

Protein Sequence (340 amino acids)

>PfGW456L13_4831 Glucosamine-6-phosphate deaminase [isomerizing], alternative (EC 3.5.99.6) (Pseudomonas fluorescens GW456-L13)
LTSKMLEEALSSFEAVQAQLQQLDPQMIEIAGRLRRQPPQVAMTVARGSSDHAASYFAYL
TMQLLGIPVASLPMSVVTMQQAPLKVSGQVAFAFSQSGQSPDLVNSLRLLRKRGALSISL
VNAEDSPLEAACEFSLPLCAGTESSVAATKSFIATLSASARLIGHWKEDTELLDACRALP
EGLRDAAQQDWSQAIDALRDCQRLMVIGRGAGFAIAQEAALKFKETSAIQAEAFSSAEVR
HGPMALIGDNYPLLVFAPRGAEQAGLLSLAADMRQRGALVLLAAPDDVLERDLTLTRAEH
PALDPILAIQSFYVMAAGLAVARGMDPDQPRHLSKVTRTH