Protein Info for PfGW456L13_4805 in Pseudomonas fluorescens GW456-L13

Annotation: Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 803 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 42 to 60 (19 residues), see Phobius details amino acids 66 to 82 (17 residues), see Phobius details amino acids 88 to 110 (23 residues), see Phobius details amino acids 122 to 141 (20 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 16 to 801 (786 residues), 1164.8 bits, see alignment E=0 PF13360: PQQ_2" amino acids 222 to 327 (106 residues), 21.2 bits, see alignment E=3e-08 amino acids 683 to 769 (87 residues), 19.7 bits, see alignment E=8.6e-08 PF01011: PQQ" amino acids 229 to 256 (28 residues), 22.8 bits, see alignment (E = 8.8e-09) amino acids 736 to 767 (32 residues), 26.9 bits, see alignment (E = 4.3e-10)

Best Hits

Swiss-Prot: 47% identical to DHG_ECOLI: Quinoprotein glucose dehydrogenase (gcd) from Escherichia coli (strain K12)

KEGG orthology group: K00117, quinoprotein glucose dehydrogenase [EC: 1.1.5.2] (inferred from 93% identity to pfo:Pfl01_4577)

MetaCyc: 47% identical to quinoprotein glucose dehydrogenase (Escherichia coli K-12 substr. MG1655)
Quinoprotein glucose dehydrogenase. [EC: 1.1.5.2]

Predicted SEED Role

"Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2)" in subsystem D-gluconate and ketogluconates metabolism or Entner-Doudoroff Pathway or Respiratory dehydrogenases 1 (EC 1.1.5.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QRC8 at UniProt or InterPro

Protein Sequence (803 amino acids)

>PfGW456L13_4805 Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2) (Pseudomonas fluorescens GW456-L13)
MSTDGALSRSRLLPTLLGILLLLMGLAMLAGGIKLSMLGGSLYYLLAGIGIALSGVLLIA
ARRAALGLYALVLFASTVWALWEVGLDWWQLVPRLALWFALGVVMLLPWFRRPLLINGPA
PMGTGALSVAVVLAGATAVASQFTHPGETFGELGRDTADMTSTAPQMPEGDWQAYGRSEF
GDRYSPLKQITPANVGKLQEAWRIQTGDLPTADDPVELTNENTPLKANGMLYACTAHSKV
LALDPDTGKELWRFDPQIKSPVGFKGFAHMTCRGVSYYDEAAYAKSESAASAVISEAGKA
VAQACPRRLYLPTADARLIALNADTGKICEGFGKNGVVDLTQGIGPFTAGGYYSTSPAAI
TRDLVIMGGHVTDNESTNEPSGVIRAFDVHDGHLVWNWDSNNPDATEPLPEGEHYSRNSA
NMWSLASVDEKLGMVYLPLGNQTPDQWGADRTPGAEKFSAGLVALDLATGKVRWNYQFTH
HDLWDMDVGSQPTLLDMKTADGVKPALIAPTKQGSLYVLDRRDGTPIIPIKEIPAPQGAV
KGDRTAPTQARSDLNLLAPELTEKAMWGASPFDQMLCRIQFKELRYEGQYTPPSEQGSLI
YPGNVGVFNWGGVSVDPVRQMLFTSPNYMAFVSKMVPRAEVAADSKRESETAGVQPNTGA
PYAVIMHPFMSPLGVPCQAPAWGYVAGIDLTTSKVVWKRKNGTSRDSSPLPIGLPLGVPS
MGGSMVTAGGVGFLSGTLDQYLRAYDVNTGNELWKSRLPAGGQATPMTYTGKDGKQYVLL
VVGGHGSLGTKMGDYVIAYKLSE