Protein Info for PfGW456L13_4772 in Pseudomonas fluorescens GW456-L13

Updated annotation (from data): ABC transporter for L-Asparagine and possibly other L-amino acids, permease component 2
Rationale: Specific phenotypes on L-Asparagine; L-Asparagine. also has weak phenotypes on other a.a., but not on aspartate; weak phenotype on glutamate; also is detrimental during growth on glutamine (along with a downstream HK-RR), which could imply that it leaks glutamate; K10002 : glutamate/aspartate transport system permease protein
Original annotation: Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 223 transmembrane" amino acids 20 to 43 (24 residues), see Phobius details amino acids 64 to 86 (23 residues), see Phobius details amino acids 92 to 111 (20 residues), see Phobius details amino acids 155 to 176 (22 residues), see Phobius details amino acids 195 to 214 (20 residues), see Phobius details TIGR01726: amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family" amino acids 12 to 116 (105 residues), 82.5 bits, see alignment E=1.3e-27 PF00528: BPD_transp_1" amino acids 33 to 221 (189 residues), 74.5 bits, see alignment E=4.7e-25

Best Hits

KEGG orthology group: K10002, glutamate/aspartate transport system permease protein (inferred from 93% identity to pfl:PFL_4873)

Predicted SEED Role

"Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4)" (TC 3.A.1.3.4)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QWA4 at UniProt or InterPro

Protein Sequence (223 amino acids)

>PfGW456L13_4772 ABC transporter for L-Asparagine and possibly other L-amino acids, permease component 2 (Pseudomonas fluorescens GW456-L13)
MEFDFSGIIPSLPGLWNGMIMTLKLMAMGVIGGIILGTILALMRLSHNKVLSNIAGAYVN
YFRSIPLLLVITWFYLAVPFVLRWITGEDTPIGAFASCIVAFMMFEAAYFCEIVRAGVQS
IPKGQMGAAQALGMSYGQMMRLIILPQAFRKMTPLLLQQSIILFQDTSLVYAVGLVDFLN
ASRASGDIIGRSNEFLIFAGLVYFIISFAASQLVKRLQKRFAV