Protein Info for PfGW456L13_4542 in Pseudomonas fluorescens GW456-L13

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 TIGR00229: PAS domain S-box protein" amino acids 12 to 122 (111 residues), 79.2 bits, see alignment E=2.9e-26 PF00989: PAS" amino acids 13 to 96 (84 residues), 46.9 bits, see alignment E=1.2e-15 PF08448: PAS_4" amino acids 17 to 119 (103 residues), 52 bits, see alignment E=3.5e-17 PF13426: PAS_9" amino acids 18 to 116 (99 residues), 45.7 bits, see alignment E=3e-15 PF08447: PAS_3" amino acids 30 to 104 (75 residues), 52.1 bits, see alignment E=2.9e-17 PF13492: GAF_3" amino acids 160 to 278 (119 residues), 28 bits, see alignment E=1.2e-09 PF13185: GAF_2" amino acids 162 to 277 (116 residues), 39.3 bits, see alignment E=3.3e-13 PF01590: GAF" amino acids 180 to 276 (97 residues), 38.7 bits, see alignment E=6.6e-13 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 285 to 443 (159 residues), 143.4 bits, see alignment E=5.5e-46 PF00990: GGDEF" amino acids 289 to 443 (155 residues), 158.1 bits, see alignment E=7.7e-50

Best Hits

KEGG orthology group: None (inferred from 82% identity to pfo:Pfl01_1323)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QSZ3 at UniProt or InterPro

Protein Sequence (448 amino acids)

>PfGW456L13_4542 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Pseudomonas fluorescens GW456-L13)
MENRNSAPLASYIDLLLDAVCAVDKQGRFVFVSAACERIFGYSADEMIGEAMIDMVHPGD
RQRTLDAAREIMGGEPKLNFENRYLRKDGSVVHILWSARWSEDDQLRIAVARDITERKQA
ESRQAALYAISEAAHAAEDLLALFKRIHMIIGEWLPALSFSVALYDQHCAQLNFPYHVDD
HEPQPEQPGTMTGRLCVEVICTGQPILLTPGQCPPLAGFEMLQTEQNAPCWLGVPLSSQN
GTIGALVVKSIPGGERYTEQDKELLQYVCAQVATAIERKQLHARLQHMAQYDQLTQLPNR
ELLRERLKASLQVAREGGGRMALLYVDLDRFKQVNDTHGHAVGDMLLQAVANRLKGCVRE
IDTVARIGGDEFVVLLHSIHAADDTDLVVEKVRQVLGQPLRLDGHSLNIQPSIGVARYPD
HGTEEKQLFRHADEAMYCAKREHHLRLV