Protein Info for PfGW456L13_4513 in Pseudomonas fluorescens GW456-L13

Annotation: HtrA protease/chaperone protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR02037: peptidase Do" amino acids 31 to 470 (440 residues), 533.8 bits, see alignment E=1.7e-164 PF00089: Trypsin" amino acids 96 to 252 (157 residues), 66.6 bits, see alignment E=7.5e-22 PF13365: Trypsin_2" amino acids 98 to 231 (134 residues), 135 bits, see alignment E=8.6e-43 PF00595: PDZ" amino acids 268 to 327 (60 residues), 39.6 bits, see alignment E=1.4e-13 amino acids 378 to 458 (81 residues), 36.8 bits, see alignment E=1e-12 PF13180: PDZ_2" amino acids 272 to 361 (90 residues), 58.8 bits, see alignment E=1.4e-19 amino acids 385 to 466 (82 residues), 39.1 bits, see alignment E=2e-13 PF17820: PDZ_6" amino acids 297 to 342 (46 residues), 40.9 bits, see alignment 3.7e-14 amino acids 410 to 461 (52 residues), 38 bits, see alignment 2.9e-13

Best Hits

Swiss-Prot: 92% identical to DEGPL_PSEF5: Probable periplasmic serine endoprotease DegP-like (mucD) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01362, [EC: 3.4.21.-] (inferred from 92% identity to pfo:Pfl01_1365)

Predicted SEED Role

"HtrA protease/chaperone protein"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QVK7 at UniProt or InterPro

Protein Sequence (474 amino acids)

>PfGW456L13_4513 HtrA protease/chaperone protein (Pseudomonas fluorescens GW456-L13)
MSIPRLKSYLSIIATVLVLGQAVTAQAVELPDFTQLVEQASPAVVNISTTQKLPDRKVNQ
QMPDLEGLPPMLREFFERGMPPQSRSPRDRQREATSLGSGFIISSDGYILTNNHVIADAD
EILVRLADRSEMKAKLIGTDPRSDVALLKIEGKDLPVLKLGKSQDLKAGQWVVAIGSPFG
FDHTVTQGIVSAVGRSLPNENYVPFIQTDVPINPGNSGGPLFNLNGEVVGINSQIYTRSG
GFMGVSFAIPIDVAMDVSNQLKSGGKVSRGWLGVVIQEVSKDLAESFGLEKPAGALVAQI
QDDGPAAKGGLQVGDVILSMNGQPIVMSADLPHLVGALKAGNKADLEVIRDGKRKNVELT
VGAIPEEGKELDSLPKSSIERSSNRLGVSVVELTDEQKKTYDLKGGVLIKEVQDGPAAMI
GLQPGDVITHLNNQAIGSTKEFADIAKALPKNRSVSMRVLRQGRASFITFKLAE