Protein Info for PfGW456L13_4467 in Pseudomonas fluorescens GW456-L13

Annotation: ATP-dependent RNA helicase HrpA (EC 3.6.4.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1303 TIGR01967: RNA helicase HrpA" amino acids 12 to 1300 (1289 residues), 2084.1 bits, see alignment E=0 PF00270: DEAD" amino acids 81 to 228 (148 residues), 30.9 bits, see alignment E=6.4e-11 PF00271: Helicase_C" amino acids 287 to 410 (124 residues), 48.6 bits, see alignment E=2.7e-16 PF04408: HA2_N" amino acids 475 to 500 (26 residues), 29 bits, see alignment (E = 2.7e-10) PF21010: HA2_C" amino acids 501 to 557 (57 residues), 35.2 bits, see alignment (E = 4.4e-12) PF07717: OB_NTP_bind" amino acids 624 to 699 (76 residues), 65.5 bits, see alignment 1.2e-21 PF11898: DUF3418" amino acids 713 to 1300 (588 residues), 676.8 bits, see alignment E=8.9e-207

Best Hits

Predicted SEED Role

"ATP-dependent RNA helicase HrpA (EC 3.6.4.13)" (EC 3.6.4.13)

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QQE5 at UniProt or InterPro

Protein Sequence (1303 amino acids)

>PfGW456L13_4467 ATP-dependent RNA helicase HrpA (EC 3.6.4.13) (Pseudomonas fluorescens GW456-L13)
MTDESPSIDKLLKNLDHAMLADRHRLRRQLLELRKKPDEARLGQWVTRMQASCEQVLARK
ASLPVIRYDDSLPIAAKRDEIKKALEKHQVLIIAGETGSGKTTQLPKICLEIGRGQHGLI
GHTQPRRIAARSVASRVAEELGTPLGALVGYQVRFEDQSDSSTLIKLMTDGILLAETQND
RYLERYDTIIVDEAHERSLNIDFLLGYLKTLLPRRPDLKVIITSATIDLERFSKHFDDAP
IVEVSGRTYPVETWYRPLTLEQDEEGNRVEDDLTVDQAILATLDEIAAYERSERRSPGDV
LVFLPGEREIRDAADMLRKAQLKHTEILPLYARLSPAEQQRIFQSHPGRRVVLATNVAET
SLTVPGIRYVIDSGTARISRYSYRAKVQRLPIEAISQASANQRKGRCGRVEPGICVRLYS
EEDFLGRPEFTDPEILRTNLAAVILQMLHLRLGEVTAFPFIEPPDGKAISDGYNLLQELS
AVDRNSQLTPLGRQLARLPVDPRMGRMLLEAAKLGSLQEVLIVASAMSIQDPRERPPERQ
QAADQAHAQWKDADSDFAGLVNLWRGFEEQRQALTASPLRNWCRKNFLNYLRLREWRDSH
RQLSLICRDMQLSLNKEPADYPKLHKAVLVGLLSQIGQKTEDGDYLGARQRRFWIHPSSG
IGKKRPQWLMTAELVETTKLYARMVAKIDADWIEPLAGHLIKKNHFEPHWEKKRGQVVAF
EQITLFGLIVVGRRPVHYGPVDPVVSRELFIREALVRGEIQSKAKCLTANKQLLEQLDEL
EAKARRRDILADEETLYAFYDARLPAEIHQTATFDSWYRINSQKDPQLLIMREEDVLARE
ASEVTAQHYPDTLHIGDLELALSYHFEPNHPRDGVTLRVPAPLLPMLPPERLEWLVPGVI
EAKCIALVRNLPKALRKNFVPVPDFVKAALQRMTFAEGSLPQALGRELLRMTGARVSDEA
WSEAAQQVESHLRMNLEIVDAQGKFLGEGRDLAELTARFAEASQAALAVPQTAKSQQPVE
PKVFAAVAEKTQQKIAGLSMTVYPALVEEAGTVKEGRFSTPAEAEFQHRRALQRLLMQQL
AEPAKFLRGKLPGLTELGLLYRDMGRVDALVEDILLASLDSCILEGEDPLPRDGAGLASL
AERKRGAWTEHAERVAKLTLEILKLWHGLQKRFKGKIDLAQAVALNDIKQQLSYLVYPGF
VRETPMQWLKELPRYLKAIEQRFEKIGAQVQRDRVWSGELSGLWTQYQTRASKHAQEGKR
DPQLELYRWWLEEYRVSLFAQQLGTKVPISDKRLNKQWSQVEA