Protein Info for PfGW456L13_4392 in Pseudomonas fluorescens GW456-L13

Annotation: Periplasmic nitrate reductase component NapE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 25 55 transmembrane" amino acids 18 to 44 (27 residues), see Phobius details PF06796: NapE" amino acids 5 to 53 (49 residues), 80.4 bits, see alignment E=2.8e-27

Best Hits

Swiss-Prot: 55% identical to NAPE_PARPN: Protein NapE (napE) from Paracoccus pantotrophus

KEGG orthology group: K02571, periplasmic nitrate reductase NapE (inferred from 69% identity to pfv:Psefu_2936)

Predicted SEED Role

"Periplasmic nitrate reductase component NapE" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QVL8 at UniProt or InterPro

Protein Sequence (55 amino acids)

>PfGW456L13_4392 Periplasmic nitrate reductase component NapE (Pseudomonas fluorescens GW456-L13)
MPSMDDSTQRVRKETRLFYFLIIVLFPLLSVAIVGGYGFFVWFLQMLLGPPGPPN