Protein Info for PfGW456L13_4390 in Pseudomonas fluorescens GW456-L13

Annotation: Periplasmic nitrate reductase precursor (EC 1.7.99.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 834 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR01706: periplasmic nitrate reductase, large subunit" amino acids 3 to 829 (827 residues), 1545.7 bits, see alignment E=0 TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 4 to 30 (27 residues), 17.4 bits, see alignment (E = 4.3e-07) PF04879: Molybdop_Fe4S4" amino acids 43 to 94 (52 residues), 63.6 bits, see alignment 2e-21 PF00384: Molybdopterin" amino acids 98 to 571 (474 residues), 241 bits, see alignment E=3.1e-75 PF01568: Molydop_binding" amino acids 717 to 825 (109 residues), 86 bits, see alignment E=2.8e-28

Best Hits

Swiss-Prot: 76% identical to NAPA_BRASB: Periplasmic nitrate reductase (napA) from Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 76% identity to bbt:BBta_1862)

MetaCyc: 68% identical to nitrate reductase large subunit (Aliivibrio fischeri)
Nitrate reductase (cytochrome). [EC: 1.9.6.1]

Predicted SEED Role

"Periplasmic nitrate reductase precursor (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4 or 1.9.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QUN6 at UniProt or InterPro

Protein Sequence (834 amino acids)

>PfGW456L13_4390 Periplasmic nitrate reductase precursor (EC 1.7.99.4) (Pseudomonas fluorescens GW456-L13)
MPMTRREFVKVQAAGIAAAAAGLPVFTSASNLITEQDMVALDWNKAPCRFCGTGCSVMVA
TKDNRVVATHGDVKAEVNRGLNCVKGYFLSKIMYGVDRLTQPLLRMTNGQYDKQGEFHPV
SWKQAFDVMELKYKEALKNKGGESIAMFGSGQWTIWEGYAANKLMKAGFRSNNIDPNARH
CMASAVMGFMRTFGIDEPMGCYDDIEVTDAFVLWGSNMAEMHPILWSRVTDRRLSQPNVK
VAVLSTFEHRSFELADIPMVFKPQTDLLILNYIANHIIESGSVNKDFVSKHTKFAKGTDD
IGYGLRADNPLEMKAKNADKANTWTDIDFEQFAAFVKPYTLERTAKETGVPTERLKALAE
LYADPKRKVVSFWTMGFNQHTRGVWANNLIYNIHLLTGKISEPGNSPFSLTGQPSACGTA
REVGTFSHRLPADLVVTNPAHRATAEKIWKLPAGTIKEKVGFHAVQQSRMLKDGVLNVYW
TMASNNMQAGPNIMQEVLPGWRNPENFVVVSDVYPTVSAQAADLILPTAMWVEKEGAYGN
AERRTQFWHQLVSAPGDAKSDLWQLVEFSKRFKTDEVWPQELLDGSPQYKGKTLYEVLFK
NGQVDQFPVEQMAAGYQNDEARDFGFYLQKGLFEEYAQFGRGHGHDLAPFDRYHSERGLR
WPVVEGKETLWRFREGYDPYVEKGSGVQFYGYPDKKAIIFALPYEPPAEAPDADFPFWLS
TGRVLEHWHTGTMTQRVEELYKAVPDALVYMHPADAKALNARRGSEVKLISRRGEMRARI
ETRGRNKPPQGLVFVPFFDANKLINKVTLDATDPISKQTDYKKCAIRIELIKTA