Protein Info for PfGW456L13_4388 in Pseudomonas fluorescens GW456-L13

Annotation: Cytochrome c-type protein NapC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02161: periplasmic nitrate (or nitrite) reductase c-type cytochrome, NapC/NirT family
  • amino acids 8 to 193 (186 residues), 337.3 bits, E=1.2e-105
• PF03264: Cytochrom_NNT
  • amino acids 18 to 192 (175 residues), 261.1 bits, E=8.6e-82

Best Hits

Swiss-Prot: 71% identical to NAPC_RHOS4: Cytochrome c-type protein NapC (napC) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

KEGG orthology group: K02569, cytochrome c-type protein NapC (inferred from 89% identity to pba:PSEBR_a2088)

MetaCyc: 58% identical to NapC (Aliivibrio fischeri)
RXN-15816 [EC: 7.1.1.8]

Predicted SEED Role

"Cytochrome c-type protein NapC" in subsystem Nitrate and nitrite ammonification or trimethylamine N-oxide (TMAO) reductase

MetaCyc Pathways

• aerobic respiration I (cytochrome c) (3/4 steps found)
• aerobic respiration II (cytochrome c) (yeast) (3/4 steps found)
• sn-glycerol 3-phosphate anaerobic respiration (2/3 steps found)
• formate to nitrite electron transfer (1/3 steps found)
• Fe(II) oxidation (3/6 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.1.1.8

Sequence Analysis Tools

See A0A293QSJ5 at UniProt or InterPro

Protein Sequence (198 amino acids)

>PfGW456L13_4388 Cytochrome c-type protein NapC (Pseudomonas fluorescens GW456-L13)
MKSLLALLKDYWAVLRRPSVHYSLGFLTLGGFIAGVIFWGGFNTALEATNTEKFCTSCHE
MRDNVFVELQETIHYTNRSGVRATCPDCHVPHEWTHKIARKMQASKEVWGKLFGTISTRD
KFLGMRRELAEHEWARLKANDSRECRNCHNFEFMDFTRQGKRAANMHSTSLASGQATCID
CHKGIAHKLPDMSGVKGW