Protein Info for PfGW456L13_4241 in Pseudomonas fluorescens GW456-L13

Annotation: Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K04719, 5,6-dimethylbenzimidazole synthase [EC: 1.14.99.40] (inferred from 93% identity to pfo:Pfl01_1643)

Predicted SEED Role

"Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.99.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QPU2 at UniProt or InterPro

Protein Sequence (216 amino acids)

>PfGW456L13_4241 Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family (Pseudomonas fluorescens GW456-L13)
MSDNAFSEAERAAVYRAIAERRDMRHFSGGTVEPELLRRLLEAAHQAPSVGLMQPWRFIR
ISDRALRGQIQALIEEERIRTAEALGERSDEFMKLKVEGINDCAEVLVAALMDDREQHIF
GRRTLPEMDMASLSCAIQNLWLASRAEGLGMGWVSLFEPQALADLLRLPPGAKPLAVLCL
GPVKEFYPAPMLVLEGWAQARPLHELLYENYWGVSQ