Protein Info for PfGW456L13_4208 in Pseudomonas fluorescens GW456-L13

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 596 PF00563: EAL" amino acids 10 to 239 (230 residues), 184.8 bits, see alignment E=2.7e-58 PF00571: CBS" amino acids 274 to 323 (50 residues), 26.3 bits, see alignment 1.1e-09 PF00990: GGDEF" amino acids 406 to 490 (85 residues), 47.7 bits, see alignment E=2.3e-16 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 418 to 497 (80 residues), 47.6 bits, see alignment E=7.8e-17

Best Hits

KEGG orthology group: None (inferred from 93% identity to pfo:Pfl01_1678)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QSL3 at UniProt or InterPro

Protein Sequence (596 amino acids)

>PfGW456L13_4208 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Pseudomonas fluorescens GW456-L13)
MTTTEQLSALSSILSQSGLHSLFQPIISLSERRILGYEALSRGPSNSPLHSPVALFAVAR
QAGRLSELEIACRKSACRRFNEQQLPGLLFLNVSPESLLEAAHQPGRTLQLLQDFGIAPS
QVVIELTEQTPTDDFQLLQTALHHYRAMGFSIALDDLGAGYSSLRLWSELRPDYVKIDRH
FIDGIHQDALKREFVGSILQIAKASRAQVIAEGIELPEELTVLTEMGVDLVQGYLLCRPQ
EHPPRDARALMPRQDSTAVALSEDGSDLSALLNELPAVNRDTPTATVLEAFRRQANLNSL
AVLDEQGQPCGIVHRHSLSDALLKPFATDLFARKPISRLMNDDFLAVELSQSLQQVSRLI
TSRARQRIEEDFIITLNGGYLGLGRVIDVLKLITELKIQQARYANPLTLLPGNVPIQQCL
TRLLQQRRESVICYVDIDSFKPFNDIYGYGRGDEVLLCLAQCLNDRVDPSRDFVGHIGGD
DFLLVLGPEDWRKRLNQLLDDFQSQCRRFYRSEHLEAGCFIAPNRQGQRQEFALLSLSIG
VVHLHPQACGQLDASQLAEMASQAKHHAKNVQGYSVHVIDSRVVSTVEEVLVSGHR