Protein Info for PfGW456L13_4197 in Pseudomonas fluorescens GW456-L13

Annotation: Potassium efflux system KefA protein / Small-conductance mechanosensitive channel

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 121 to 144 (24 residues), see Phobius details amino acids 175 to 192 (18 residues), see Phobius details amino acids 203 to 225 (23 residues), see Phobius details amino acids 244 to 260 (17 residues), see Phobius details amino acids 271 to 292 (22 residues), see Phobius details amino acids 319 to 345 (27 residues), see Phobius details amino acids 352 to 373 (22 residues), see Phobius details amino acids 394 to 417 (24 residues), see Phobius details amino acids 437 to 459 (23 residues), see Phobius details amino acids 480 to 502 (23 residues), see Phobius details amino acids 509 to 537 (29 residues), see Phobius details PF21088: MS_channel_1st" amino acids 483 to 524 (42 residues), 35.4 bits, see alignment 1.3e-12 PF00924: MS_channel_2nd" amino acids 526 to 590 (65 residues), 60.1 bits, see alignment E=2.8e-20 PF21082: MS_channel_3rd" amino acids 597 to 682 (86 residues), 26.2 bits, see alignment E=1.3e-09

Best Hits

KEGG orthology group: None (inferred from 89% identity to pfo:Pfl01_1688)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QSK3 at UniProt or InterPro

Protein Sequence (700 amino acids)

>PfGW456L13_4197 Potassium efflux system KefA protein / Small-conductance mechanosensitive channel (Pseudomonas fluorescens GW456-L13)
MTLLSVAPAQAAGLPGLLNNQAKAQPEAQEPLGQSLDEVIKSLENDQQRTKLLADLKRLR
DTTKQAQPITEEGVLGLIGGTLANFEKKFSGVDSPLTLWSSEFDQAREELVALALPANEW
LPMLFAFAMILMIWSLLAAALIWISHRVRMRFGLTEELPQHPRALDMLRFALRKLGPWLI
ALVITVYMSYALPSSLGKSLAMVLAYALVVGTCFSAICVIAFSLLDGPHRHRALYILRHQ
AFRPLWLIGSFAAFGEALSDPQLVNSLGSHLAHTAATAANVLAALSTGRFILRFRRPIAH
LIRNQPLSRRLTRRALNDTLSILGTFWYVPALVLVAISLFATFISAGDTSTALRQSLICT
VLLVLCMVINGLVRRHALKPQRGIRRYAVYSERLKNFFYTIVHLLVWLAFIELGLRVWGM
SLIRFTEGDGHEVSVKLFSLAGTLLFAWLIWILSDTAVHHALTRSRKGLANARAQTMMPL
IRNVLFIAIFIIALIVALANMGMNVTPLLAGAGVIGLAIGFGAQSLVADLITGLFIIIED
SLAIDDYVDVGGHLGTVEGLTIRTVRLRDIDGIVHTIPFSEIKSIKNYSREFGYAIFRVA
VPANMDIDNAIKLMREVGQKMRTDPLQRRNIWSPLEFQGVESFESGNAILRARFKTAPIK
QWEVSRAFNLSLKRHMDEAGLDLATPRMSVHIMTPGVAQE