Protein Info for PfGW456L13_4103 in Pseudomonas fluorescens GW456-L13

Updated annotation (from data): Nitrate reductase (EC 1.7.99.4)
Rationale: Specifically important for utilizing Sodium nitrate. Automated validation from mutant phenotype: the predicted function (1.7.99.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: Assimilatory nitrate reductase large subunit (EC:1.7.99.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 905 PF04879: Molybdop_Fe4S4" amino acids 5 to 52 (48 residues), 59.3 bits, see alignment 6e-20 PF00384: Molybdopterin" amino acids 75 to 488 (414 residues), 240.3 bits, see alignment E=6.6e-75 PF01568: Molydop_binding" amino acids 587 to 695 (109 residues), 87 bits, see alignment E=1.8e-28 PF04324: Fer2_BFD" amino acids 848 to 895 (48 residues), 31.3 bits, see alignment 4.3e-11

Best Hits

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 89% identity to pfo:Pfl01_1779)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QPH1 at UniProt or InterPro

Protein Sequence (905 amino acids)

>PfGW456L13_4103 Nitrate reductase (EC 1.7.99.4) (Pseudomonas fluorescens GW456-L13)
MDRQITASTCCYCGVGCGVLIEHDGEHILGVSGDPAHPANFGKLCSKGSTLHLTGDLTAR
ALYPELRLGKSLARHRTDWDTALDHATSVFAATIAEHGPDSVAFYISGQLLTEDYYAFNK
LARALVGTNNIDSNSRLCMSSAVVGYKRSLGADAPPCSYEDLELSDCVMIVGSNMAYAHP
VLFRRLEAAKSRRPQMKVIVIDPRRTDTCDLADLHLAILPGTDVALFHGILHLLLWEDWI
DRDFIRDHTEGLAELKNLVRDYTPQMVSQLCGISLGQLQQCAEWIGTSPSFLSLWCMGLN
QSTAGSAKNSALINLHLATGQIGRPGAGPFSLTGQPNAMGGRETGSLSNLLPGHRDAANA
QHRAQVASYWGVEQLPESPGLTAIELFEQVRNGKIKALWIACTNPAQSMPDQTAVRAALQ
ACPFVVLQEAFSTTETAKFADLLLPAASWGEKEGCVTNSERRISHVRQAIVAPGEARPDW
AITVDFAQRLEKHLRPGQASLFEFDNPAQVFDEYKQLTQGRDLDLSGISHALIDRLGPQQ
WPFPAGAVEGTARLYVDGVFATANGRAQFVADPYRAAKEQRDARFPLTLITGRLRDQWHG
MSRTGTAAQLFGHVSEAELSLHPDELRRHRLQPGDLVSLKSRRGAIVVAVASDDSVRPGQ
AFLPMHWGDRFLKGGVNSLTLPAFDPLSKQPELKHSGVRLDPVDLPWKLFALIEGDVQRH
FETLRPLCEAFSYVSLSLVGRERPALLVRAANTSAPSAELLSEIDKNLALDDGPVLAYDD
PRRAIGKRVRIENGRITAIRLAGETLAQHWLQGLWLEGRADQQLRRWLLAPLSAPPGNLD
MPFNSNKILCNCKNVGQNAICAGISRGLDLQGLKQELGCGTQCGSCVPEIKRLLAATAQP
VTVFS