Protein Info for PfGW456L13_4094 in Pseudomonas fluorescens GW456-L13

Annotation: 23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 756 PF22020: RlmL_1st" amino acids 6 to 61 (56 residues), 55.8 bits, see alignment 8.9e-19 PF02926: THUMP" amino acids 70 to 156 (87 residues), 58.2 bits, see alignment E=2.3e-19 PF01170: UPF0020" amino acids 165 to 380 (216 residues), 143.5 bits, see alignment E=1.7e-45 PF10672: Methyltrans_SAM" amino acids 515 to 708 (194 residues), 70.6 bits, see alignment E=3.4e-23 PF03602: Cons_hypoth95" amino acids 590 to 678 (89 residues), 30.2 bits, see alignment E=8.5e-11

Best Hits

Swiss-Prot: 96% identical to RLMKL_PSEPF: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K12297, ribosomal RNA large subunit methyltransferase L [EC: 2.1.1.173] (inferred from 96% identity to pfo:Pfl01_1788)

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QU40 at UniProt or InterPro

Protein Sequence (756 amino acids)

>PfGW456L13_4094 23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-) (Pseudomonas fluorescens GW456-L13)
MSDRFELFLTCPKGLEGLLIEEAVGLGLEEAREHTSAVRGMATMETAYRLCLWSRLANRV
LLVLKRFQMKDAEDLYHGVLDIEWQDHMLNDGTLAVEFSGHGSGIDNTHFGALKVKDAIV
DKLRTPQGDRPSIDKLNPDLRIHLRLDRGEAILSLDLSGHSLHQRGYRLQQGAAPLKENL
AAAILIRSGWPRIAAEGGALADPMCGVGTFLVEAAMIAADMAPNLRREQWGFTAWLGHVP
ALWKKLHEEATERAAAGLAKPPLWIRGYEADPRLIQPGRNNVERAGLSEWIKIYQGEVAT
FEPRPDQNQKGLVICNPPYGERLGDEASLLYLYQNLGERLRQACLNWEAAVFTGAPDLGK
RMGIRSHKQYSFWNGALPCKLLLIKVLPDQFVTGERRTPEQRQVEREQAAYDQAPNEPQE
RKFNKNGNPIKPAPAPAPVVEQPRLSEGGQMFANRLQKNLKALGKWVKREGIDCYRVYDA
DMPEYSMAIDLYHDWVHVQEYVAPKSIDPEKASARMFDALAAIPQALNIDKSRVVVKRRE
RQSGTKQYERQAAQGKFVEVNEGGVKLLVNLTDYLDTGLFLDHRPMRMRIQKEAAGKRFL
NLFCYTATASVHAAKGGARSTTSVDLSKTYLDWARRNLSLNGFSDKNRLEQGDVMAWLEA
SRDEYDMIFIDPPTFSNSKRMEGVFDVQRDQVQLIDLAMARLAPGGVLYFSNNFRKFELE
ANLGERYAVEEITAQTIDPDFARNAKIHRAWKITAR