Protein Info for PfGW456L13_3931 in Pseudomonas fluorescens GW456-L13

Annotation: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 266 PF03328: HpcH_HpaI" amino acids 18 to 243 (226 residues), 199.1 bits, see alignment E=3.2e-63

Best Hits

Swiss-Prot: 54% identical to HPCH_KLEP7: 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K02510, 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase [EC: 4.1.2.-] (inferred from 89% identity to pba:PSEBR_a2976)

MetaCyc: 53% identical to 4-hydroxy-2-ketopimelate aldolase monomer (Escherichia coli C)
4-HYDROXY-2-KETOPIMELATE-LYSIS-RXN [EC: 4.1.2.52]

Predicted SEED Role

"2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4)" (EC 4.1.2.n4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.2.- or 4.1.2.52 or 4.1.2.n4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QRE0 at UniProt or InterPro

Protein Sequence (266 amino acids)

>PfGW456L13_3931 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4) (Pseudomonas fluorescens GW456-L13)
MNMPHNAFKAALNSSGTQYGIWAGFATGYAAEIVATTGYDWMLIDGEHAPNTVPGVLAQL
QAVAPYSTAPVVRAVNGDANLIKQLLDIGAQTLMIPMVETAAQAQALVRAMRYPPHGIRG
VGGGLTRATRWDDVPDYLNTAHEQLCLIVQVESRTGVENVAAIAAVEGVDAVFIGPADLS
IGLGHAGNPGHPEVQERIQYAVNATLAAGKACGILAPNEEDARRYQRWGCQFIAVAIDIS
LLRQGAMASLARYREPAATQAPSRTY