Protein Info for PfGW456L13_3930 in Pseudomonas fluorescens GW456-L13

Annotation: Gluconate dehydratase (EC 4.2.1.39)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 93% identical to RHMD_DELAS: L-rhamnonate dehydratase (rhmD) from Delftia acidovorans (strain DSM 14801 / SPH-1)

KEGG orthology group: K12661, L-rhamnonate dehydratase [EC: 4.2.1.90] (inferred from 96% identity to pba:PSEBR_a2975)

MetaCyc: 81% identical to L-rhamnonate dehydratase (Sphingomonas sp. SKA58)
L-rhamnonate dehydratase. [EC: 4.2.1.90]

Predicted SEED Role

"Gluconate dehydratase (EC 4.2.1.39)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.39)

MetaCyc Pathways

Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
Entner-Doudoroff pathway II (non-phosphorylative) (6/9 steps found)
L-rhamnose degradation II (3/8 steps found)
L-rhamnose degradation III (2/7 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.39 or 4.2.1.90

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QSW5 at UniProt or InterPro

Protein Sequence (394 amino acids)

>PfGW456L13_3930 Gluconate dehydratase (EC 4.2.1.39) (Pseudomonas fluorescens GW456-L13)
MGIPTIKHVRAFTLRGGGADYHDQADGHWIDDHIATPMSKYPEYRQSRRTFGINVLGTLV
VEIEASDGTVGFAVTTGGEPAAYIVEKHLARFLEGVRVTDIEKIWDQMYQSTLYYGRKGI
VINTISGVDLALWDLLGKIRQEPVHQLLGGAVRDELQFYATGARPDLAQKMGFIGGKMPL
HHGPAEGEEGLRKNLEELATMRERVGPDFWLMLDCWMSLDLNYATKLAVGAHEYGLKWIE
EALPPDDYWGYAALRNNVPKGMLVTTGEHEATRWGFRMLLEMGCCDIIQPDVGWCGGITE
LVKISALADAHNALVIPHGSSVYSYHFVATRHNSPFAEFLMMAPKADEVVPMFHPQLLGE
PVPVNGRMRLSALDQPGFGVTLNPECQLHRPYNR