Protein Info for PfGW456L13_3927 in Pseudomonas fluorescens GW456-L13

Annotation: Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 943 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details transmembrane" amino acids 907 to 919 (13 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 6 to 33 (28 residues), 19.1 bits, see alignment (E = 6.2e-08) PF02738: MoCoBD_1" amino acids 265 to 484 (220 residues), 123.9 bits, see alignment E=6.3e-40 PF20256: MoCoBD_2" amino acids 516 to 602 (87 residues), 37.6 bits, see alignment E=1.8e-13 amino acids 764 to 879 (116 residues), 55.4 bits, see alignment E=7.3e-19

Best Hits

KEGG orthology group: None (inferred from 94% identity to pfo:Pfl01_1905)

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QTZ8 at UniProt or InterPro

Protein Sequence (943 amino acids)

>PfGW456L13_3927 Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16) (Pseudomonas fluorescens GW456-L13)
MSNRDISRRSFLQGGLVAGVSVTLTPLSSQALAALMENSVTVPSEQWLGNNGKARARNDA
LSKVCGSKVFARDIRAKDMPGWPQQQGHAMLLKTIKADHIFAGIDLSWLGAELQPDRIVT
AEDLVKDGIVFPEEHAPDPLLPAGKVPMFIGHPVAILIWNDFERFRQAKNQLKFNDKAIR
YGAQVPFYEGDPYGSFRYVRVGGATSADEDEFASLKDSILFPMLKNRRPVWNSQPNLHGN
LTERGLFYAERMKQQIDTPPDNWLVFDERYKTPSIEPAALEPDNGNGWYDPATKTLHFVV
ATQCPLETATETAKMIAPSRFGLANLNMHPGYTVGYGSKDHNIFVYYAALAALYGAGVPV
RLANDRYEQFQSGIKRHPFDIRYQLAVDKNDHSFKIFRSEMSVDGGGRINYSPSVAAVGA
TAAQSIYYMPQNDLQVTAYHSRAVEAGSMRGYGTLQSMAATEMMVDEIAGRLGVDAIELR
RKNALRSGMKNTQGAIPAGALRLHEILDKAAVHEVWKNREAIKKQREAADPDNWYGVGFA
ICQKDFGTGSEAPMASIEFTAEGRISLRHIGIEIGTGMSTSQAMVVADFLGVPAHEVKTG
ETEWKELQLVSGGNPYIMSQAEQDNFLRNPRWVGKVASASSATNSAYYFSHATREAARVL
FNHGLWPAALEIWRQGPYGGQANPYVVRREDAHWVDGKLTANGMEPLPFAMLAKRVHERG
LVSGATVHGFNRWSWAEAEFSIDGVRERLPLDGLAVKYGDGAPKAKKAQMNSAGFHLLDR
QGIAYPATQLNNAAVTYYSPVATLVEVKVNKGSNEVSVLNHHSWVECGRVLVEELVRGQL
EGGIAMGIGHALMEEMPLYEGGPGEGDWNFNRYRLPMARHVAVWKQTAEILPPLSPSDPS
KGIAEVVMIPVVGAIGNAVAHAIGKRVRDLPITSARIKEALNG