Protein Info for PfGW456L13_3819 in Pseudomonas fluorescens GW456-L13
Annotation: Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 73% identical to XYLX_PSEPU: Toluate 1,2-dioxygenase subunit alpha (xylX) from Pseudomonas putida
KEGG orthology group: K05549, benzoate 1,2-dioxygenase alpha subunit [EC: 1.14.12.10] (inferred from 88% identity to pfl:PFL_3858)MetaCyc: 73% identical to XylX (Pseudomonas putida mt-2)
Predicted SEED Role
"Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10)" in subsystem Benzoate degradation (EC 1.14.12.10)
MetaCyc Pathways
- benzoate degradation I (aerobic) (2/2 steps found)
- meta cleavage pathway of aromatic compounds (6/10 steps found)
- mandelate degradation to acetyl-CoA (10/18 steps found)
- toluene degradation IV (aerobic) (via catechol) (6/13 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)
- superpathway of aerobic toluene degradation (13/30 steps found)
- superpathway of aromatic compound degradation via 2-hydroxypentadienoate (15/42 steps found)
KEGG Metabolic Maps
- Benzoate degradation via CoA ligation
- Benzoate degradation via hydroxylation
- Fluorobenzoate degradation
Isozymes
Compare fitness of predicted isozymes for: 1.14.12.10
Use Curated BLAST to search for 1.14.12.10
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A293QR23 at UniProt or InterPro
Protein Sequence (454 amino acids)
>PfGW456L13_3819 Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10) (Pseudomonas fluorescens GW456-L13) MTLRPEYLHSLLEDDPEQGLYRCKREMFTDPRLFDLEMEHIFEGNWLYLAHESQIPNIND FYTTTMGRQSIFIARNKDGVLNAFINACSHRGATLCRHKSGNKSSYTCPFHGWTFNNSGK LLKVKDPANAGYPSSFNCEGSHDLTKVARFESYRGFLFGSLKADVLPLVEHLGESAKIID MIVDQSADGLEVLRGSSSYIYEGNWKLTAENGADGYHVSSVHWNYAATQNQRKQREAGDT NPTMSAGSWAKQGGGFYSFDKGHMLLWTRWANPEDRPLYERRDELAQDFGKARADWMIEN SRNLCLYPNVYLMDQFSSQIRIARPISVNRTEITIYCIAPKGESDHARSSRIRQYEDFFN VSGMATPDDLEEFRSCQTSYQGSVTTWNDMSRGAEHWIEGADEAAKEIDLHPLLSGVRTE DEGLFVLQHKYWQQTMLKALAAEQSKLIAVEDVQ