Protein Info for PfGW456L13_3780 in Pseudomonas fluorescens GW456-L13

Annotation: Choline-sulfatase (EC 3.1.6.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 519 PF00884: Sulfatase" amino acids 14 to 357 (344 residues), 193.1 bits, see alignment E=7.3e-61

Best Hits

KEGG orthology group: K01130, arylsulfatase [EC: 3.1.6.1] (inferred from 76% identity to mex:Mext_0526)

Predicted SEED Role

"Choline-sulfatase (EC 3.1.6.6)" in subsystem Choline Transport or Choline and Betaine Uptake and Betaine Biosynthesis (EC 3.1.6.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.6.1, 3.1.6.6

Use Curated BLAST to search for 3.1.6.1 or 3.1.6.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QRD7 at UniProt or InterPro

Protein Sequence (519 amino acids)

>PfGW456L13_3780 Choline-sulfatase (EC 3.1.6.6) (Pseudomonas fluorescens GW456-L13)
MAFPGLTLAADKAPNILVIMGDDVGWSNIGVYNQGMMAGRTPNLDQLANEGMRFTDYYAE
ASCTAGRANFITGELPIRTGMTTVGQAGSPVGIPAEAVTIATALKAMGYATGQFGKNHLG
DLNQFLPTVHGFDEFFGYLYHLDAMEDPAHPNYPKELLDSVGPRNMVHSWASTTDDTTVM
PRWGKVGKQKIEDAGTLYPERMKTVDEEIRDKAFSFIDKAKQDNKPFFVWLNPTRMHIVT
HLSDKYEAMRNSQNGWSEQEAGMAQLDDIVGDVLAKLKKDGMDDNTIVIFTTDNGAENFT
WPDGGTTPFAMGKGTVMEGGFRVPAIIRWPGKVPANQIANGIMSGMDWFPTLVAAAGNPN
ITAELLKGKQLGDTTYKVHLDGYDQTPMITGKGPSNRHEVFYFGESSLGAVRIDDYKYRF
IDQPGGWMGAKVAVDVPILTNLRLDPFERMGWPENQAAQGSQQYFDWFKFQFWRFVFVQQ
QVAKLAETAIEFPPMQKGASFNLDSVKAKIAAARAEMAK