Protein Info for PfGW456L13_3707 in Pseudomonas fluorescens GW456-L13

Annotation: Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF19335: HMBD" amino acids 53 to 79 (27 residues), 40.9 bits, see alignment (E = 5.6e-14) PF16576: HlyD_D23" amino acids 114 to 321 (208 residues), 244.8 bits, see alignment E=2.1e-76 PF16572: HlyD_D4" amino acids 159 to 211 (53 residues), 83.9 bits, see alignment 2e-27 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 203 to 397 (195 residues), 133 bits, see alignment E=5.8e-43 PF13533: Biotin_lipoyl_2" amino acids 216 to 246 (31 residues), 26.3 bits, see alignment (E = 1.8e-09) PF13437: HlyD_3" amino acids 217 to 318 (102 residues), 70.4 bits, see alignment E=6.9e-23 PF11604: CusF_Ec" amino acids 425 to 488 (64 residues), 73.4 bits, see alignment E=4.2e-24

Best Hits

KEGG orthology group: K07798, Cu(I)/Ag(I) efflux system membrane protein CusB (inferred from 62% identity to psa:PST_2082)

Predicted SEED Role

"Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QT22 at UniProt or InterPro

Protein Sequence (494 amino acids)

>PfGW456L13_3707 Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family (Pseudomonas fluorescens GW456-L13)
MILEKWNGALLVGVSLALGVAGGYWFAHPRMSEVPVTAREQKINSPDERKALYWYDPMYP
QQKFDKPGKSPFMDMQLVPRYASGVEDRATVSIDPGLTQNLGLRFATVTRGVFDSSLDVT
GVLAFNERDVAVIQARTAGFVERVYAHAPGDLLKANAALADILVPEWAAAQTEFLALKRN
GDADLLAAARQRLRLTGMPAALISQVERSGKVQTNLTISSPISGVLQELNVRAGMTVAAG
ETLARVNGLSRVWLAVAVPESDAGAVTVGQAVEARLPAFPGTMFNGKVSAILPETNPDSR
TLRVRVELPNPEGRLRPGLTAQVRLSHSTGHSVLWVPSEAVIRTGRRALVMLAEDAGRYR
PVEVQPGQESDGKTEILKGLEEGRKVVTSGQFLLDSEASLKGIVATSVQESPASGAAASL
HEADGQIVEINDKEVTLAHGPFKTLGMPGMTMTFPLASAALMQGLKAGDKVRVAVSQTDD
GLRVERLAKSGSQP