Protein Info for PfGW456L13_3513 in Pseudomonas fluorescens GW456-L13

Annotation: Coenzyme PQQ synthesis protein E

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 366 TIGR02109: coenzyme PQQ biosynthesis enzyme PqqE" amino acids 1 to 345 (345 residues), 601.3 bits, see alignment E=5.4e-185 PF04055: Radical_SAM" amino acids 7 to 163 (157 residues), 91.9 bits, see alignment E=8.2e-30 PF13353: Fer4_12" amino acids 11 to 86 (76 residues), 25.6 bits, see alignment E=2.1e-09 PF13186: SPASM" amino acids 234 to 299 (66 residues), 40.1 bits, see alignment E=5.3e-14 TIGR04085: radical SAM additional 4Fe4S-binding SPASM domain" amino acids 242 to 326 (85 residues), 31.3 bits, see alignment E=2.1e-11

Best Hits

Swiss-Prot: 82% identical to PQQE_PSEF5: PqqA peptide cyclase (pqqE) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K06139, pyrroloquinoline quinone biosynthesis protein E (inferred from 83% identity to pfo:Pfl01_5161)

MetaCyc: 73% identical to glutamate Cgamma--tyrosine C3 ligase (Klebsiella pneumoniae)
RXN-11176 [EC: 1.21.98.4]

Predicted SEED Role

"Coenzyme PQQ synthesis protein E" in subsystem Coenzyme PQQ synthesis or Pyrroloquinoline Quinone biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.21.98.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QSJ7 at UniProt or InterPro

Protein Sequence (366 amino acids)

>PfGW456L13_3513 Coenzyme PQQ synthesis protein E (Pseudomonas fluorescens GW456-L13)
LWLLAELTYRCPLQCPYCSNPLDFARHGEELSTEEWIRVFREARDMGAAQLGFSGGEPLV
RQDLAELIKAARDMGYYTNLITSGIGLTEQKVRDFKVAGLDHIQISFQAADEAVNNMLAG
SRKAFAQKLAMARAVKAQGYPMVLNFVTHRHNIDRIAQIIELCLELEADFVELATCQFYG
WAELNRVGLLPTREQLQRAERITNEYRQRLETQGHPCKLIFVTPDYYEERPKTCMNGWAN
LFLDITPDGTALPCHSARQLPVAFPNVREHSLSHIWNDSFGFNRFRGDEWMKEPCRSCDE
KHKDLGGCRCQAFMLTGDASNADPVCSKSPHHGVILKAREEAEVPGMGIEHLTLRNAKTS
QLIYRG