Protein Info for PfGW456L13_3503 in Pseudomonas fluorescens GW456-L13

Updated annotation (from data): Beta-ureidopropionase (EC 3.5.1.6)
Rationale: Specifically important for utilizing Cytosine; Uridine. Automated validation from mutant phenotype: the predicted function (3.5.1.6) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: Beta-ureidopropionase (EC 3.5.1.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 TIGR01879: amidase, hydantoinase/carbamoylase family" amino acids 18 to 414 (397 residues), 444 bits, see alignment E=2.4e-137 PF04389: Peptidase_M28" amino acids 72 to 179 (108 residues), 28.6 bits, see alignment E=1.7e-10 PF01546: Peptidase_M20" amino acids 89 to 414 (326 residues), 108.3 bits, see alignment E=7.8e-35 PF07687: M20_dimer" amino acids 221 to 321 (101 residues), 25.8 bits, see alignment E=1.3e-09

Best Hits

Swiss-Prot: 48% identical to HYUC_RHIML: N-carbamoyl-L-amino-acid hydrolase (hyuC) from Rhizobium meliloti

KEGG orthology group: K06016, N-carbamoyl-L-amino-acid hydrolase [EC: 3.5.1.87] (inferred from 92% identity to pfo:Pfl01_3439)

MetaCyc: 48% identical to N-carbamoyl-L-amino-acid hydrolase subunit (Sinorhizobium meliloti CECT4114)
N-carbamoyl-L-amino-acid hydrolase. [EC: 3.5.1.87]

Predicted SEED Role

"Beta-ureidopropionase (EC 3.5.1.6)" in subsystem Hydantoin metabolism or L-2-amino-thiazoline-4-carboxylic acid-Lcysteine conversion or Pyrimidine utilization (EC 3.5.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.87

Use Curated BLAST to search for 3.5.1.6 or 3.5.1.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293Q2N5 at UniProt or InterPro

Protein Sequence (427 amino acids)

>PfGW456L13_3503 Beta-ureidopropionase (EC 3.5.1.6) (Pseudomonas fluorescens GW456-L13)
MNAAVDVLQSSHQHINRDRLWQSLMELARLGATVKGGVCRLALTDLDRQARDIFVKWCID
AGCTVSIDAVGNIFARRPGRNPDLPPVMTGSHIDTQPTGGKFDGCFGVLAGVEVLRTLND
LNIETEAPLEVVVWTNEEGSRFAPCMMGSGVFAEKFTLEETLAKVDAEGVTVGQALNAIG
YAGPRKVSGHKVGAYFEAHIEQGPILEDERKTIGVVMGALGQKWFDLKLRGVEAHAGPTP
MHLRKDALVGAAVIVGALNRAALSHQPHACGTVGCLQAYPGSRNVIPGEVRMTLDFRHLE
PARLDSMIAEVREVIETTCDEHGLTFELTPTADFPPLYFDKGCVEAVRGAAQSLGLSHMD
IVSGAGHDAIFLAELGPAGMIFVPCEGGISHNEIENAAPEDLAAGCAVLLRAMLAASAAI
AKGELTA