Protein Info for PfGW456L13_3394 in Pseudomonas fluorescens GW456-L13

Annotation: 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00108: Thiolase_N
  • amino acids 4 to 259 (256 residues), 251.2 bits, E=1.7e-78
• TIGR01930: acetyl-CoA C-acyltransferase
  • amino acids 6 to 388 (383 residues), 464.1 bits, E=1.7e-143
• PF00109: ketoacyl-synt
  • amino acids 83 to 119 (37 residues), 23.9 bits, 4.5e-09
• PF02803: Thiolase_C
  • amino acids 268 to 389 (122 residues), 161.3 bits, E=1.2e-51

Best Hits

Swiss-Prot: 45% identical to PAAJ_ECOLI: 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase (paaJ) from Escherichia coli (strain K12)

KEGG orthology group: K00632, acetyl-CoA acyltransferase [EC: 2.3.1.16] (inferred from 73% identity to bxe:Bxe_C0597)

MetaCyc: 46% identical to beta-ketoadipyl-CoA thiolase (Pseudomonas sp. Y2)
RXN0-6512 [EC: 2.3.1.223]; 3-oxoadipyl-CoA thiolase. [EC: 2.3.1.223, 2.3.1.174]

Predicted SEED Role

"3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9)" (EC 2.3.1.16, EC 2.3.1.9)

MetaCyc Pathways

• oleate β-oxidation (30/35 steps found)
• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• catechol degradation III (ortho-cleavage pathway) (6/6 steps found)
• fatty acid salvage (6/6 steps found)
• adipate degradation (5/5 steps found)
• superpathway of phenylethylamine degradation (9/11 steps found)
• superpathway of salicylate degradation (6/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (11/14 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• L-isoleucine degradation I (5/6 steps found)
• 3-oxoadipate degradation (2/2 steps found)
• phenylacetate degradation I (aerobic) (7/9 steps found)
• valproate β-oxidation (7/9 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (4/5 steps found)
• adipate biosynthesis (4/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• ketolysis (2/3 steps found)
• polyhydroxybutanoate biosynthesis (2/3 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• acetoacetate degradation (to acetyl CoA) (1/2 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• ketogenesis (3/5 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• 4-methylcatechol degradation (ortho cleavage) (4/7 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (4/7 steps found)
• 5,6-dehydrokavain biosynthesis (engineered) (6/10 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (5/9 steps found)
• glycerol degradation to butanol (10/16 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
• fatty acid β-oxidation VII (yeast peroxisome) (2/5 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• (2S)-ethylmalonyl-CoA biosynthesis (1/4 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• androstenedione degradation I (aerobic) (16/25 steps found)
• acetyl-CoA fermentation to butanoate (3/7 steps found)
• benzoyl-CoA degradation I (aerobic) (3/7 steps found)
• pyruvate fermentation to butanoate (3/7 steps found)
• ethylbenzene degradation (anaerobic) (1/5 steps found)
• isopropanol biosynthesis (engineered) (1/5 steps found)
• pyruvate fermentation to acetone (1/5 steps found)
• 2-deoxy-D-ribose degradation II (3/8 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• mandelate degradation to acetyl-CoA (10/18 steps found)
• superpathway of testosterone and androsterone degradation (17/28 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
• L-lysine fermentation to acetate and butanoate (4/10 steps found)
• superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (4/10 steps found)
• 10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) (1/6 steps found)
• 4-ethylphenol degradation (anaerobic) (1/6 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• 2-methylpropene degradation (2/8 steps found)
• L-tryptophan degradation III (eukaryotic) (7/15 steps found)
• mevalonate pathway I (eukaryotes and bacteria) (1/7 steps found)
• mevalonate pathway II (haloarchaea) (1/7 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)
• 3-phenylpropanoate degradation (3/10 steps found)
• methyl tert-butyl ether degradation (3/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• 4-oxopentanoate degradation (2/9 steps found)
• isoprene biosynthesis II (engineered) (1/8 steps found)
• mevalonate pathway III (Thermoplasma) (1/8 steps found)
• mevalonate pathway IV (archaea) (1/8 steps found)
• L-glutamate degradation VII (to butanoate) (3/12 steps found)
• ethylmalonyl-CoA pathway (2/11 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (4/16 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (4/17 steps found)
• superpathway of aerobic toluene degradation (13/30 steps found)
• toluene degradation VI (anaerobic) (4/18 steps found)
• jasmonic acid biosynthesis (4/19 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
• androstenedione degradation II (anaerobic) (9/27 steps found)
• sitosterol degradation to androstenedione (1/18 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (18/42 steps found)
• platensimycin biosynthesis (6/26 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (3/22 steps found)
• superpathway of L-lysine degradation (17/43 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (19/47 steps found)
• superpathway of ergosterol biosynthesis I (3/26 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)
• superpathway of cholesterol biosynthesis (3/38 steps found)
• superpathway of cholesterol degradation III (oxidase) (10/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Ethylbenzene degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Lysine degradation
• Propanoate metabolism
• Pyruvate metabolism
• Synthesis and degradation of ketone bodies
• Terpenoid biosynthesis
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.16, 2.3.1.174, 2.3.1.9

Use Curated BLAST to search for 2.3.1.16 or 2.3.1.174 or 2.3.1.223 or 2.3.1.9

Sequence Analysis Tools

See A0A293QS75 at UniProt or InterPro

Protein Sequence (391 amino acids)

>PfGW456L13_3394 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9) (Pseudomonas fluorescens GW456-L13)
MKDIVIVDAVRTPIGKFRGSLSGVRADHLGSLVISRLLERVDVSPTLVDDVIFGNVTQIG
EQSANIARTALLGAGWPVTVAGLTIDRKCGSGEVAVHMAVGAIAAGAADIVVAGGAENMS
RVPMGSNREIHGAAFGWMAAQRYELTSQGEAAERMADKWALSRDALDDFAFASHQRAAAA
CDAGYFDNETIPVVVEELREKELSEPAGVLRHDETIRRDTSREKLSTLKTSFRPDTGRIT
AGNSSQISDGAAALLLMSADTAKKLGLKARARVVAFTTVGSDPTLMLTGPIAATQKVLAK
AGLSINDIDLFEVNEAFASVPLAWMQETGVPHSKLNVNGGAIALGHPLGASGARLMTTML
NELERRGGRYGLQAICCAGGMGTATIIERLD