Protein Info for PfGW456L13_338 in Pseudomonas fluorescens GW456-L13

Annotation: Glycogen phosphorylase (EC 2.4.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 816 transmembrane" amino acids 73 to 84 (12 residues), see Phobius details TIGR02093: glycogen/starch/alpha-glucan phosphorylases" amino acids 13 to 813 (801 residues), 1097.5 bits, see alignment E=0 PF00343: Phosphorylase" amino acids 100 to 813 (714 residues), 1008.2 bits, see alignment E=1.1e-307

Best Hits

Swiss-Prot: 48% identical to PHSG_SYNY3: Glycogen phosphorylase (glgP) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 96% identity to pfo:Pfl01_0349)

Predicted SEED Role

"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QIQ7 at UniProt or InterPro

Protein Sequence (816 amino acids)

>PfGW456L13_338 Glycogen phosphorylase (EC 2.4.1.1) (Pseudomonas fluorescens GW456-L13)
MTQEPLVREAEVAAFRDAVLTKLTYAVGKDPDHAFDHDWFEAIALAARDHMVEHWMDHTR
QIYRKGQKRVYYLSLEFLIGRLLFDSLSNLGLLDVAREALTELGVDLERIRMLEPDAALG
NGGLGRLAACFLESMSTLGIAGHGYGIRYEHGLFRQAIVDGWQQEQTEHWLDFGNPWEFE
RPEVAYTIGFGGGVETLTDDTGKSRQVWSPAETVRAIAYDTPVVGWRGASVNTLRLWRAR
AMEDLHLERFNAGDHLGAVAEVARAESISRVLYPADSTEAGQELRLRQEYFFVAASLQDL
LRRHRNMHTSVLTLGDHASIQLNDTHPSIAVAELMRQLVDVYDVAWDAAWQVTVDTLSYT
NHTLLPEALETWPVGLMERMLPRHMQIIYLINAHHIDSLRAKGIHDFDVLRAVSLIEEDN
GRRVRMGNLAFLGSHSINGVSGLHTQLMRQTVFSELHKLYPERINNKTNGITFRRWLYQA
NPELTSMLVDALGPEVLDNPEERLLALEPFAEKSAFRKAFAEQRLHSKKALAYLIHERLG
VAVNPAAMFDVQVKRIHEYKRQLLNLMHTVALYQAIRAEPEIDWVPRVKIFAGKAAASYH
QAKLIIKLTNDIARVVNNDPTVRGLLKVVFLPNYNVSLAESIIPAADLSEQISTAGFEAS
GTSNMKFGLNGALTIGTLDGANVEMSERIGTEHMFIFGLSAQQVEARKQNHEFSAVPDIA
ASHRLNDVLQAIRGGVFSPDDPSRYAGLIDSLVDYDRFMVCADFDSYWHAQMRVEAHWHD
SKEWWRSAVLNTARMGWFSSDRTIREYATEIWKALE