Protein Info for PfGW456L13_3340 in Pseudomonas fluorescens GW456-L13

Annotation: Hydroxyacylglutathione hydrolase (EC 3.1.2.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 255 TIGR03413: hydroxyacylglutathione hydrolase" amino acids 4 to 255 (252 residues), 339.2 bits, see alignment E=6.6e-106 PF00753: Lactamase_B" amino acids 14 to 88 (75 residues), 48.4 bits, see alignment E=1.1e-16 amino acids 109 to 167 (59 residues), 28.4 bits, see alignment E=1.6e-10 PF16123: HAGH_C" amino acids 168 to 255 (88 residues), 93.7 bits, see alignment E=7.4e-31

Best Hits

Swiss-Prot: 84% identical to GLO2_PSEPF: Hydroxyacylglutathione hydrolase (gloB) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01069, hydroxyacylglutathione hydrolase [EC: 3.1.2.6] (inferred from 84% identity to pfo:Pfl01_2184)

Predicted SEED Role

"Hydroxyacylglutathione hydrolase (EC 3.1.2.6)" in subsystem Glutathione: Non-redox reactions or Methylglyoxal Metabolism (EC 3.1.2.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QMB2 at UniProt or InterPro

Protein Sequence (255 amino acids)

>PfGW456L13_3340 Hydroxyacylglutathione hydrolase (EC 3.1.2.6) (Pseudomonas fluorescens GW456-L13)
MIQITALPAFTDNYIWLLQDHGSKRCAVVDPGDAGPVQAWLAANPGWVLSDILITHHHHD
HVGGVEQLKKATDAKVYGPASETIPARDVALKDNDKVCVLGWDFDVLAVPGHTLGHIAYY
HHGLLFCGDTLFAAGCGRLFEGTPQQMYSSLTRLAGLPEDTLVYCTHEYTLGNLKFAAAV
EPNNPHTAERLAKVTRQRESGIMTLPSTLALEKLTNPFLRSGETSVKEKADERTGTDNSA
PDMVFAALRAWKDRF