Protein Info for PfGW456L13_3330 in Pseudomonas fluorescens GW456-L13
Annotation: NAD kinase (EC 2.7.1.23)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 99% identical to NADK_PSEPF: NAD kinase (nadK) from Pseudomonas fluorescens (strain Pf0-1)
KEGG orthology group: K00858, NAD+ kinase [EC: 2.7.1.23] (inferred from 99% identity to pba:PSEBR_a3277)Predicted SEED Role
"NAD kinase (EC 2.7.1.23)" in subsystem NAD and NADP cofactor biosynthesis global (EC 2.7.1.23)
MetaCyc Pathways
- superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (7/8 steps found)
- cytosolic NADPH production (yeast) (4/5 steps found)
- mitochondrial NADPH production (yeast) (4/5 steps found)
- NADP biosynthesis (1/1 steps found)
- NAD phosphorylation and transhydrogenation (1/2 steps found)
- NAD phosphorylation and dephosphorylation (1/3 steps found)
- NAD(P)/NADPH interconversion (3/6 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.7.1.23
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0F4U1Y1 at UniProt or InterPro
Protein Sequence (296 amino acids)
>PfGW456L13_3330 NAD kinase (EC 2.7.1.23) (Pseudomonas fluorescens GW456-L13) MEQFRNIGIIGRLGSSQVLDTVRRLKRFLLDRHLHVILEDTIAEVLPGHGLQTSSRKMLG EVCDMVIVVGGDGSLLGAARALAKHNIPVLGINRGSLGFLTDIRPDELETKVAEVLDGHY LVENRFLLQAEVRRHGEAIGQGDALNDVVLHPGKSTRMIEFELYIDGQFVCSQKADGLIV ATPTGSTAYALSAGGPIMHPKLDAIVIVPMYPHTLSGRPIVVDGNSELKIVVSKDMQIYP QVSCDGQNHFTCAPGDTITVSKKAQKLRLIHPLDHNYYEVCRTKLGWGSKLGGGGD