Protein Info for PfGW456L13_330 in Pseudomonas fluorescens GW456-L13

Updated annotation (from data): Formiminoglutamic iminohydrolase (EC 3.5.3.13)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (FORMIMINOGLUTAMATE-DEIMINASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: Formiminoglutamic iminohydrolase (EC 3.5.3.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 454 TIGR02022: formiminoglutamate deiminase" amino acids 1 to 453 (453 residues), 743.8 bits, see alignment E=3.3e-228 PF22429: HutF_N" amino acids 1 to 44 (44 residues), 57.4 bits, see alignment 1.5e-19 PF01979: Amidohydro_1" amino acids 48 to 429 (382 residues), 113.1 bits, see alignment E=2.6e-36 PF07969: Amidohydro_3" amino acids 207 to 429 (223 residues), 40.2 bits, see alignment E=5e-14

Best Hits

KEGG orthology group: K05603, formimidoylglutamate deiminase [EC: 3.5.3.13] (inferred from 95% identity to pfo:Pfl01_0357)

MetaCyc: 90% identical to formiminoglutamate deiminase subunit (Pseudomonas fluorescens)
Formimidoylglutamate deiminase. [EC: 3.5.3.13]

Predicted SEED Role

"Formiminoglutamic iminohydrolase (EC 3.5.3.13)" in subsystem Histidine Degradation (EC 3.5.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QE12 at UniProt or InterPro

Protein Sequence (454 amino acids)

>PfGW456L13_330 Formiminoglutamic iminohydrolase (EC 3.5.3.13) (Pseudomonas fluorescens GW456-L13)
MSAFFAERALLPSGWANNVRLEVSADGVLTHIQADSNADGAERLSGPLLPGMPNLHSHAF
QRAMAGLAEVAGNPNDSFWTWRDLMYRLVGKISPEQLGVIARQLYIEMLKAGYTSVAEFH
YVHHDISGQPYADPAELALRISQAAGSAGIGLTLLPVLYSHSGFGGQTPNEGQRRFINST
ENYLKLQSRLQPLLAQQPAQALGLCFHSLRAVTPQQISEVLAASDKQCPVHIHIAEQQKE
VDDCLTWSGRRPLQWLYENVEVDQRWCLVHATHANPEEVTSMARSRAIAGLCLTTEANLG
DGIFPAVDFLAQGGRMGIGSDSHVSLSVVEELRWLEYGQRLRDQRRNRLYGADQPMVGRT
LFDAALDGGAQALGQPIGALEVGKRADWLVLDGNDPYLATANGDGILNRWLFAGGDRQVR
DVLVNGQWVVRDGRHADEEESNRAFTQVLRELLG