Protein Info for PfGW456L13_3298 in Pseudomonas fluorescens GW456-L13

Annotation: NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1632 PF21075: GDH_ACT1" amino acids 35 to 178 (144 residues), 160.5 bits, see alignment E=9.2e-51 PF21073: GDH_HM1" amino acids 344 to 403 (60 residues), 81.8 bits, see alignment (E = 1.1e-26) PF21076: GDH_ACT2" amino acids 407 to 496 (90 residues), 113.1 bits, see alignment 3e-36 PF21079: GDH_HM2" amino acids 504 to 546 (43 residues), 44.5 bits, see alignment (E = 4.9e-15) PF21077: GDH_ACT3" amino acids 552 to 631 (80 residues), 91 bits, see alignment 1.2e-29 PF21078: GDH_HM3" amino acids 648 to 711 (64 residues), 97.1 bits, see alignment (E = 2.1e-31) PF05088: Bac_GDH_CD" amino acids 733 to 1227 (495 residues), 896.1 bits, see alignment E=2.2e-273 PF21074: GDH_C" amino acids 1272 to 1609 (338 residues), 429.7 bits, see alignment E=3.3e-132

Best Hits

Swiss-Prot: 83% identical to DHE2_PSEAE: NAD-specific glutamate dehydrogenase (gdhB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Predicted SEED Role

"NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293Q238 at UniProt or InterPro

Protein Sequence (1632 amino acids)

>PfGW456L13_3298 NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form (Pseudomonas fluorescens GW456-L13)
MAFFTAASKADFQHQLQAALAQHISEQALPQVALFSEQFFGIISLDELTQRRLSDLAGCT
LSAWRMLERFDHAQPQVRVYNPDYERHGWQSTHTAVEVLHHDLPFLVDSVRTELNRRGYS
IHTLQTTVLSVRRGSKGELLEILPKGTQGDDIQQESLMYLEIDRCANTAELNVLSKELEQ
VLGEVRVAVGDFEPMKAKVQDILTKLDNSQFAVDGDEKNEIKSFLEWLVGNHFTFLGYEE
FVVSDEADGGHIVYDKDSFLGLTKLLRAGLTYDDLRIEDYAVNYLREPTLLSFAKAAHPS
RVHRPAYPDYVSIREIDADGKVIKECRFMGLYTSSVYGESVRVIPYIRRKVEEIERRSGF
QAKAHLGKELAQVLEVLPRDDLFQTPVDELFSTVMSIVQIQERNKIRVFLRKDPYGRFCY
CLAYVPRDIYSTEVRQKIQQVLMDRLKASDCEFWTFFSESVLARVQLILRVDPKNRLDID
PLLLEKEVVQACRSWQDDYAALTVESFGEAHGTNVLADFPKGFPAGYRERFAAHSAVVDM
QHLLSLNEKNPLVMSFYQPLGQVSGQRMLHCKLYHADTPLALSDVLPILENLGLRVLGEF
PYRLRHNSGREFWIHDFAFTAAEGLELDIQQLNDTLQDAFVHIVRGDAENDGFNRLVLTA
GLPWRDVALLRAYARYMKQIRLGFDLGYIASTLNNHTDIAHELTRLFKTRFYLARKLTGE
DLGDKQQRLEHAILSALDDVQVLNEDRILRRYLDLIKATLRTNFYQTDANGHNKSYFSFK
FNPHLIPELPKPVPKFEIFVYSPRVEGVHLRFGNVARGGLRWSDREEDFRTEVLGLVKAQ
QVKNSVIVPVGAKGGFLPRRLPLGGSRDEIAAEGIACYRIFISGLLDITDNLKDGALVPP
ANVVRHDDDDPYLVVAADKGTATFSDIANGIAIDYGFWLGDAFASGGSAGYDHKKMGITA
KGAWVGVQRHFRERGINVQEDSITVVGVGDMAGDVFGNGLLMSDKLQLVAAFNHLHIFID
PNPEPASSFAERQRLFDLPRSAWSDYDTSLMSEGGGIFSRSAKSIAISPQMQERFDIQAD
KLTPTELLNALLKAPVDLLWNGGIGTYVKASSESHADVGDKANDALRVNGNELRCKVVGE
GGNLGMTQLGRVEFGLNGGGSNTDFIDNAGGVDCSDHEVNIKILLNEVVQAGDMTDKQRN
ELLASMTDEVGGLVLGNNYKQTQALSLAARRALPRIAEYKRLMNDLEGRGKLDRAIEFLP
AEEAINERIAEGHGLTRAELSVLISYSKIDLKEQLLGSLVPDDDYLTRDMETAFPPTLVS
KFSAAMRRHRLKREIVSTQIANDLVNHMGITFVQRLKESTGMSPANVAGAYVIVRDIFHL
PHWFRQIENLDYKVSADVQLELMDELMRLGRRATRWFLRARRNEQNAARDVAHFGPHLAA
LGLKLDELLSGEIRETWQTRYQAYVEAGVPELLARMVAGTTHLYTLLPIIEASDVTGQDP
ADVAKAYFAVGSALDITWYLQQISALPVENNWQALAREAFRDDVDWQQRAITISVLQQGD
GSQEVETRLDLWMQQHESMIERWRAMLVEIRAASGTDYAMYAVANRELLDLALSGQAVVP
AAVAAAELEPAA