Protein Info for PfGW456L13_321 in Pseudomonas fluorescens GW456-L13
Annotation: Histidine ammonia-lyase (EC 4.3.1.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 45% identical to HUTH_SYMTH: Histidine ammonia-lyase (hutH) from Symbiobacterium thermophilum (strain T / IAM 14863)
KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 92% identity to pfo:Pfl01_0365)MetaCyc: 40% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]
Predicted SEED Role
"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)
MetaCyc Pathways
- L-histidine degradation II (5/5 steps found)
- L-histidine degradation VI (7/8 steps found)
- L-histidine degradation I (4/4 steps found)
- L-histidine degradation III (4/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.3
Use Curated BLAST to search for 4.3.1.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A293QJ41 at UniProt or InterPro
Protein Sequence (507 amino acids)
>PfGW456L13_321 Histidine ammonia-lyase (EC 4.3.1.3) (Pseudomonas fluorescens GW456-L13) MSQAEKIVITESQVSWQDVVAVARHGAQLELSAPIWARIENAQAIVQRIVTSGERAYGIN TGLGALCNVSLQDEQLSQLSRNTLLSHACGVGVPLTDEQTRSIMCAAIVNYSHGKSGLHR QVVEALLALLNRGITPQVPSQGSVGYLTHMAHIGIALLGVGNVSYRGQIVSAQQALAEEG LQPVKLGAKDGLCLVNGTPCMSGLSCLAIADATRLLEWADVIGAMSFEAQRGQIDAFDAE IIALKPHPGMQQVGINLRALLDGSEVIAQSKGIRTQDALSIRSIPQVHGAARDQLVHAIK QIETELNGATDNPLVLGTADNYRVVSQANPHGQSVAMAADLLAIAMAEIGSIAERRLDRL INPHVSGLPAFLVANPGVNSGMMIVQYVAASLCAENRQLAQPAVLDNYVTSGLQEDHLSL GTNAALKLHRALENCTQILAIEYLLAAQAFEFLKEQRFGAGTDTAWRLLRERVPAYDQDR WLAPDIAAAASVLKDPVLLQKTLPNLN