Protein Info for PfGW456L13_316 in Pseudomonas fluorescens GW456-L13

Updated annotation (from data): N-formylglutamate deformylase (EC 3.5.1.68)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (3.5.1.68) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: N-formylglutamate deformylase (EC 3.5.1.68)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 267 TIGR02017: N-formylglutamate deformylase" amino acids 4 to 262 (259 residues), 395.4 bits, see alignment E=6.1e-123 PF05013: FGase" amino acids 13 to 230 (218 residues), 248.7 bits, see alignment E=3.9e-78

Best Hits

KEGG orthology group: K01479, formiminoglutamase [EC: 3.5.3.8] (inferred from 93% identity to pfo:Pfl01_0368)

MetaCyc: 76% identical to N-formylglutamate amidohydrolase (Pseudomonas putida)
N-formylglutamate deformylase. [EC: 3.5.1.68]

Predicted SEED Role

"N-formylglutamate deformylase (EC 3.5.1.68)" in subsystem Histidine Degradation (EC 3.5.1.68)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.68 or 3.5.3.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QIN4 at UniProt or InterPro

Protein Sequence (267 amino acids)

>PfGW456L13_316 N-formylglutamate deformylase (EC 3.5.1.68) (Pseudomonas fluorescens GW456-L13)
VDKVLNFKQGRVPLLISMPHAGVRLTPAVEAGLIPDAKSLPDTDWHIPQLYDFAAELGAS
TLAAEYSRFVIDLNRPSDDKPLYVGATTGLYPATLFDGIPLFREGLEPSKEERATYLEQI
WTPYHSTLQQELARLKAEFGYALLFDAHSIRSIIPHLFDGKLPDFNLGTFNGASCDPQLA
TRLEAICALHDNYTHVLNGRFKGGHITRHYGNPAENIHAVQLELGQCTYMEEFEPFRYRP
DLAEPTRLVLKELLQGLLAWGEKHYKR