Protein Info for PfGW456L13_3061 in Pseudomonas fluorescens GW456-L13

Annotation: Multidrug efflux membrane fusion protein MexE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 417 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 38 to 368 (331 residues), 264.8 bits, see alignment E=4.5e-83 PF16576: HlyD_D23" amino acids 62 to 287 (226 residues), 54.3 bits, see alignment E=1.8e-18 PF13533: Biotin_lipoyl_2" amino acids 62 to 110 (49 residues), 46.5 bits, see alignment 3.8e-16

Best Hits

Swiss-Prot: 33% identical to BEPF_BRUSU: Efflux pump periplasmic linker BepF (bepF) from Brucella suis biovar 1 (strain 1330)

KEGG orthology group: None (inferred from 94% identity to pfo:Pfl01_2659)

Predicted SEED Role

"Multidrug efflux membrane fusion protein MexE"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QLJ8 at UniProt or InterPro

Protein Sequence (417 amino acids)

>PfGW456L13_3061 Multidrug efflux membrane fusion protein MexE (Pseudomonas fluorescens GW456-L13)
MEQSLKHLRFPLALLAVVVISACGKAPETAASMPAAKVSVAKVLEQPVNEWDEFTGRLEA
PETVEIRPRVSGQIDEVAFTEGALVKKGDLLFQIDPRPFQAEVRRLEALVAQSRANATRS
ENEAQRGERLRTSNAISAELADSRTSAAQEARAAVGALQAQLDLARLNLSFTRVTAPISG
RVSRAEITAGNLVTADTTPLTSVVSTDKVYAYFDADERVFLKYTELARQGKRGATTPVYM
GLSNEDGNPHQGVMNFVDNQVNPKTGTIRGRAVFDNSDGSYTPGLYARLKLVGSGTYSAM
LINDEAVGTDLGKKFVLVMDADNKTAYRAVELGPKIEGLRIVRNGLNKDDTIIVKGLQRV
RPGSPVTPEVIPMASEQTLAALAQQRQALEASNLPQVAPARVAPGTAVKLAATTPRG