Protein Info for PfGW456L13_2941 in Pseudomonas fluorescens GW456-L13
Annotation: Glycerate kinase (EC 2.7.1.31)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 52% identical to GLXK_BACSU: Glycerate kinase (glxK) from Bacillus subtilis (strain 168)
KEGG orthology group: K00865, glycerate kinase [EC: 2.7.1.31] (inferred from 89% identity to pfo:Pfl01_2908)MetaCyc: 81% identical to glycerate 2-kinase (Pseudomonas simiae)
GKI-RXN [EC: 2.7.1.165]
Predicted SEED Role
"Glycerate kinase (EC 2.7.1.31)" in subsystem Allantoin Utilization or D-galactarate, D-glucarate and D-glycerate catabolism or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Glycine and Serine Utilization or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle (EC 2.7.1.31)
MetaCyc Pathways
- superpathway of glycol metabolism and degradation (6/7 steps found)
- superpathway of D-glucarate and D-galactarate degradation (4/5 steps found)
- D-glucarate degradation I (3/4 steps found)
- D-galactarate degradation I (3/4 steps found)
- glycolate and glyoxylate degradation I (3/4 steps found)
- 2-O-α-mannosyl-D-glycerate degradation (1/2 steps found)
- Entner-Doudoroff pathway II (non-phosphorylative) (6/9 steps found)
- photorespiration I (6/9 steps found)
- photorespiration II (6/10 steps found)
- photorespiration III (5/9 steps found)
- 2-deoxy-D-ribose degradation II (3/8 steps found)
- formaldehyde assimilation I (serine pathway) (6/13 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (14/31 steps found)
KEGG Metabolic Maps
- Glycerolipid metabolism
- Glycine, serine and threonine metabolism
- Glyoxylate and dicarboxylate metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.31
Use Curated BLAST to search for 2.7.1.165 or 2.7.1.31
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A293QLA9 at UniProt or InterPro
Protein Sequence (378 amino acids)
>PfGW456L13_2941 Glycerate kinase (EC 2.7.1.31) (Pseudomonas fluorescens GW456-L13) MKIVIAPDSFKDSLSAQGVADAIALGLAQVWPDAQLIKCPMADGGEGTVESILAACEGQQ RRTLVRGPLGATVEAAWGWLPQSHTAIIEMAEASGLQLVPVAQRDACISSTFGTGQLIRA ALDAGAQRVILAIGGSATNDGGAGAMQALGVKLLDAQGQTLSPGGLALGQLARVDLSEID PRLAQVRFDIAADVNNPLCGPHGASAIFGPQKGASPVQVEQLDRALGHFADHCARALNND VRDEPGSGAAGGLGFAAKAFLGAQFTAGVEVVAELVGLADAVAGADLVITGEGRFDAQTL RGKTPFGVARVAREHGVPVIVIAGTLGEGYQALYEHGIDAAFALASGPMTLEQACAEAPR LLRERATDVARVWRLASR