Protein Info for PfGW456L13_2912 in Pseudomonas fluorescens GW456-L13

Annotation: Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 PF03060: NMO" amino acids 8 to 344 (337 residues), 257 bits, see alignment E=6.6e-80 PF04481: DUF561" amino acids 155 to 244 (90 residues), 24.8 bits, see alignment E=2.3e-09 PF01070: FMN_dh" amino acids 165 to 262 (98 residues), 27 bits, see alignment E=4.6e-10

Best Hits

Swiss-Prot: 42% identical to NMO_PSESP: Nitronate monooxygenase (pnoA) from Pseudomonas sp.

KEGG orthology group: K00459, nitronate monooxygenase [EC: 1.13.12.16] (inferred from 87% identity to pfo:Pfl01_2940)

Predicted SEED Role

"Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9)" in subsystem Fatty Acid Biosynthesis FASII (EC 1.3.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.9

Use Curated BLAST to search for 1.13.12.16 or 1.3.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QQZ1 at UniProt or InterPro

Protein Sequence (355 amino acids)

>PfGW456L13_2912 Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9) (Pseudomonas fluorescens GW456-L13)
MNQWPDTRILDLLGIELPIIQGPMAGATNSSMVIATCNAGGLGSMPAAMLSTDQLREELK
TIRQHTRRPINVNFFCHRPPAPDEQRAQDWKNLLQPYYQELGADFDAPTPVSNRAPFNNA
ACEVVEAFRPEVVSFHFGLPEKSLLDRVKATGAKVLSSATTVAEAVWLEQHGCDAIIAMG
YEAGGHRGMFLSDDLSSQVGTFALVPQVVDAVKVPVIAAGGIADARGVAAAFMLGASAVQ
VGTAYLFTPEAKVSPSHHKALRTARESETAVTNVFTGRPARGILNRVMRELGPMSAKAPA
FPFAGGALMPLRAKGEADFSNLWAGQAFTLGVELTTEALTRKLAEEGLARLLGRP