Protein Info for PfGW456L13_281 in Pseudomonas fluorescens GW456-L13

Annotation: Type IV pilus biogenesis protein PilM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 TIGR01175: type IV pilus assembly protein PilM" amino acids 9 to 353 (345 residues), 449.5 bits, see alignment E=3.8e-139 PF11104: PilM_2" amino acids 14 to 353 (340 residues), 453.8 bits, see alignment E=5e-140 PF14450: FtsA" amino acids 196 to 326 (131 residues), 48.2 bits, see alignment E=2.1e-16

Best Hits

KEGG orthology group: K02662, type IV pilus assembly protein PilM (inferred from 88% identity to pfo:Pfl01_0405)

Predicted SEED Role

"Type IV pilus biogenesis protein PilM" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293PWI1 at UniProt or InterPro

Protein Sequence (354 amino acids)

>PfGW456L13_281 Type IV pilus biogenesis protein PilM (Pseudomonas fluorescens GW456-L13)
VPGLFKKKSNTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPVGAVIEKNIAELEGV
GLALTRALAKARTALKNVAVAVAGSAVITKTIEMDAGLCEDEMESQLKIEADQYIPYPLD
EVAIDFEVQGTSGRNPERVNVLLAACRKENVEVREAALALAGLTARVVDVEAYALERSFE
LLASRLAVSEKPLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAQKKGGLPDDYAAEVLQPFREALVQQVSRSLHFFLASGQYSAVDHVLLAGGTASI
PGLDRLIEQQLGTPTQVANPFCEMTLGSKVNGAALASDAPALMIACGLALRSFD