Protein Info for PfGW456L13_2740 in Pseudomonas fluorescens GW456-L13
Updated annotation (from data): Anthranilate 1,2-dioxygenase (deaminating, decarboxylating) (EC 1.14.12.1)
Rationale: Specifically important for utilizing L-Tryptophan. Automated validation from mutant phenotype: the predicted function (1.14.12.1-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 76% identical to ANTDA_ACIAD: Anthranilate 1,2-dioxygenase large subunit (antA) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
KEGG orthology group: K05599, anthranilate 1,2-dioxygenase (deaminating, decarboxylating) large subunit [EC: 1.14.12.1] (inferred from 83% identity to pfs:PFLU5194)MetaCyc: 76% identical to anthranilate dioxygenase oxygenase component large subunit (Acinetobacter)
Anthranilate 1,2-dioxygenase (deaminating, decarboxylating). [EC: 1.14.12.1]
Predicted SEED Role
"Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10)" in subsystem Benzoate degradation (EC 1.14.12.10)
MetaCyc Pathways
- benzoate degradation I (aerobic) (2/2 steps found)
- anthranilate degradation I (aerobic) (1/1 steps found)
- 2-nitrobenzoate degradation II (2/3 steps found)
- meta cleavage pathway of aromatic compounds (6/10 steps found)
- mandelate degradation to acetyl-CoA (10/18 steps found)
- toluene degradation IV (aerobic) (via catechol) (6/13 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)
- superpathway of aerobic toluene degradation (13/30 steps found)
- superpathway of aromatic compound degradation via 2-hydroxypentadienoate (15/42 steps found)
KEGG Metabolic Maps
- Benzoate degradation via CoA ligation
- Benzoate degradation via hydroxylation
- Carbazole degradation
- Fluorobenzoate degradation
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.14.12.1, 1.14.12.10
Use Curated BLAST to search for 1.14.12.1 or 1.14.12.10
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A293QQV2 at UniProt or InterPro
Protein Sequence (461 amino acids)
>PfGW456L13_2740 Anthranilate 1,2-dioxygenase (deaminating, decarboxylating) (EC 1.14.12.1) (Pseudomonas fluorescens GW456-L13) MNSAKSITQWQEYIQGCLDFRPAEGIYRVARDMFTEPELFDLEMELIFEKNWIYACHESE IANPNDFLTMRAGRQPMIITRDGNNQLHALINACQHRGATLTRVSKGNQSTFTCPFHAWC YKSDGRLVKVKAPGEYPEGFDKATRGLKKARIESYKGFVFISLDVNGSDSLEDYLGDAKV FFDMMVAQSPTGELEILPGKSTYSYDGNWKLQHENGLDGYHVSTVHYNYVSTVQHRQQVN AANGGVSDTLDYSKLGAGDAETDDGWFSFKNGHSLLFSDMPNPTVRAGYATVMPRLIEEY GQQQAEWMMHRLRNLNIYPSLFFMDQISSQLRIVRPVAWNKTEITSQCIGVKGESDADRE NRIRQFEDFFNVSGMGTPDDLVEFREAQRGFQARLERWNEVSRGSEKWVEGPTPNSEVLG INPVLTGTEFTHEGLYINQHGSWQRFLLQGLEQKALKLKEV