Protein Info for PfGW456L13_2575 in Pseudomonas fluorescens GW456-L13
Annotation: O-acetylhomoserine sulfhydrylase (EC 2.5.1.49)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 93% identity to pfo:Pfl01_3679)MetaCyc: 42% identical to 4-methylaminobutanoate oxidase (Alicycliphilus denitrificans)
RXN-13065 [EC: 1.5.3.21]
Predicted SEED Role
"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49)" in subsystem Methionine Biosynthesis (EC 2.5.1.49)
MetaCyc Pathways
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
- L-methionine biosynthesis III (4/4 steps found)
- L-homocysteine biosynthesis (2/2 steps found)
- N-methylpyrrolidone degradation (2/3 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
- superpathway of L-cysteine biosynthesis (fungi) (3/6 steps found)
- S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)
- S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (2/5 steps found)
- nicotine degradation I (pyridine pathway) (6/17 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.49
Use Curated BLAST to search for 1.5.3.21 or 2.5.1.49
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A293QP56 at UniProt or InterPro
Protein Sequence (463 amino acids)
>PfGW456L13_2575 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) (Pseudomonas fluorescens GW456-L13) MRPFWLDQALKADASETCPPLEGDARADVCIVGGGYTGLWTAIMLKEQNPELDVLLIEAD ICGAGASGRNGGCALSWSAKYFTLERLFGVEEAVRLVKESERSIHAIGAFCEQYGVDADY RLDGTLYTATNRAQVGSTDAVIAALERNGINSFTQRPVADVQRMAGSSKHLEGWFSPAAA SVQPGKLVRGLRRVALQLGVRIHENTAMTGLEEGRPAGIQTPKGTIRADRVVLAMNAWMA RAFPQFERSVAIVSSDMLITEPRPDLLDEIGLTSGVTVLDSRIFVHYYHNTPDGRIMLGK GGNTFAYGGRMLPVFDQPSPYAGLLQSSLADFFPAFADVKVEATWNGPSDRSVTGLPFFG QMSASGNVFYGFGYSGSGVGPCHMGGQILASMVQGLDNPWTRSPLVNGPLGFFPPEPIRY IGSLMVRNAIRRKERAEDHGHRPRHLDVRLAKFAAAAGKADKG