Protein Info for PfGW456L13_256 in Pseudomonas fluorescens GW456-L13

Annotation: Permeases of the major facilitator superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 426 transmembrane" amino acids 39 to 60 (22 residues), see Phobius details amino acids 73 to 93 (21 residues), see Phobius details amino acids 105 to 124 (20 residues), see Phobius details amino acids 130 to 151 (22 residues), see Phobius details amino acids 162 to 182 (21 residues), see Phobius details amino acids 191 to 213 (23 residues), see Phobius details amino acids 244 to 265 (22 residues), see Phobius details amino acids 277 to 296 (20 residues), see Phobius details amino acids 308 to 325 (18 residues), see Phobius details amino acids 331 to 354 (24 residues), see Phobius details amino acids 366 to 389 (24 residues), see Phobius details amino acids 395 to 414 (20 residues), see Phobius details PF07690: MFS_1" amino acids 43 to 379 (337 residues), 148.7 bits, see alignment E=3.2e-47 amino acids 268 to 414 (147 residues), 38.7 bits, see alignment E=9.2e-14 PF00083: Sugar_tr" amino acids 71 to 219 (149 residues), 39.7 bits, see alignment E=4.4e-14

Best Hits

Swiss-Prot: 64% identical to YNFM_ECOLI: Inner membrane transport protein YnfM (ynfM) from Escherichia coli (strain K12)

KEGG orthology group: K08224, MFS transporter, YNFM family, putative membrane transport protein (inferred from 91% identity to pfo:Pfl01_0416)

Predicted SEED Role

"Permeases of the major facilitator superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QDU0 at UniProt or InterPro

Protein Sequence (426 amino acids)

>PfGW456L13_256 Permeases of the major facilitator superfamily (Pseudomonas fluorescens GW456-L13)
VKTAVAPLAHEVSPSAPDAIAAELAEIYIEKGTPAFMRTVLALFCGGFATFALLYCVQPM
MPLLSDEYSINAAQSSLILSIATGMLAIGLLITGPISDRIGRKPVMVAALFAAALCTMAS
AMMPSWHGVLLMRALIGLSLSGLAAVAMTYLSEEIHPQHIGLAMGLYIGGNAIGGMSGRL
ITGVLIDFVSWHTAMLVIGGLALIAAAVFWKILPESRNFRARSLHPRSLLDGFTMHFRDA
GLPLLFLEAFVLMGAFVTLFNYIGYRLLAAPYHMEQAFVGLLSVVYLSGIYSSAKIGSLA
DQLGRRKVLWSTIALMIGGLALTMFTPLPLVIIGMLIFTFGFFGAHSVASSWIGRRATRA
KGQASSLYLFSYYAGGSIAGTVGGVFWHLGGWNGIGLFIGTLLVIALLIALKLAKLPLLP
QNTSAT