Protein Info for PfGW456L13_2510 in Pseudomonas fluorescens GW456-L13

Annotation: Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 PF00355: Rieske" amino acids 20 to 102 (83 residues), 72.3 bits, see alignment E=2.6e-24 PF19298: KshA_C" amino acids 122 to 323 (202 residues), 260.9 bits, see alignment E=1.1e-81

Best Hits

Swiss-Prot: 48% identical to KSHA5_RHORH: 3-ketosteroid-9-alpha-monooxygenase, oxygenase component (kshA) from Rhodococcus rhodochrous

KEGG orthology group: None (inferred from 67% identity to cti:RALTA_B0514)

Predicted SEED Role

"Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit" in subsystem Phenylpropanoid compound degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QQ30 at UniProt or InterPro

Protein Sequence (347 amino acids)

>PfGW456L13_2510 Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit (Pseudomonas fluorescens GW456-L13)
MSTLHRIEAKLLEDRYARGWHCLGLAAQYRDGKAHRLEVFGTRLVAFQGEDGELHILDGY
CPHMGADLSTGCVEGNSIRCPFHAWRWGADGVCDDIPYAKRIPPRAKIKSWPIMEENQLL
FVWNDPEGNPPLPEQRIPRIDACYSDEWAAWEVAELQIDTNCRELIDNIADMAHFGTVHG
APVDEFINIFQGHLATQIMRARSERLSGDSVLTTEATYFGPAYQITEMNGAMNGQPIHSI
LLNCHVPVDLDSFTLRYGVLVKKIPGLSEEQNQAMAKAYVAQAQAAFYEDVAIWHSKTRV
DNPLLCDGDGPVYQLRRWYEQFYTDVEALSADVAEHKKFVVRASERV