Protein Info for PfGW456L13_2503 in Pseudomonas fluorescens GW456-L13

Annotation: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (EC 3.7.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00561: Abhydrolase_1
  • amino acids 28 to 134 (107 residues), 82.7 bits, E=5.2e-27
• PF12697: Abhydrolase_6
  • amino acids 29 to 263 (235 residues), 79 bits, E=1.3e-25
• PF12146: Hydrolase_4
  • amino acids 30 to 257 (228 residues), 58.9 bits, E=7.4e-20

Best Hits

KEGG orthology group: None (inferred from 60% identity to reu:Reut_A1513)

MetaCyc: 46% identical to 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase (Comamonas testosteroni)
RXN-12718 [EC: 3.7.1.17]

Predicted SEED Role

"2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (EC 3.7.1.-)" in subsystem Biphenyl Degradation or Central meta-cleavage pathway of aromatic compound degradation or carbazol degradation cluster (EC 3.7.1.-)

MetaCyc Pathways

• androstenedione degradation I (aerobic) (16/25 steps found)
• superpathway of testosterone and androsterone degradation (17/28 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (18/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (19/47 steps found)

KEGG Metabolic Maps

• 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
• Ascorbate and aldarate metabolism
• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biphenyl degradation
• Caprolactam degradation
• Ethylbenzene degradation
• Inositol phosphate metabolism
• Limonene and pinene degradation
• Nicotinate and nicotinamide metabolism
• Phenylalanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.7.1.- or 3.7.1.17

Sequence Analysis Tools

See A0A293QMH2 at UniProt or InterPro

Protein Sequence (275 amino acids)

>PfGW456L13_2503 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (EC 3.7.1.-) (Pseudomonas fluorescens GW456-L13)
MGASAQGHFVTLPDGLQLHYKDTGNGEPVIFIHGSGPGASGHSNFKQNYPLFTEAGYRVI
VPDLPGYGASDKPDTLYTLDFFVAALSGLLDALDIQRCVLVGNSLGGAIAIKLALDQPQR
ISRLVLMAPGGLMEKEQYYLQMEGIQKMGAAFAKGELNDAAGMRRLLALQLFDETLISDE
TVNERVAVVKQQPVCVLSTMQVPNMTSRLGELQCPILGFWGMNDKFCPSSGAQTMLEHCT
QIRFVMLSECGHWVQVEHRDFFNRQCLAFFQEARG